4.2 Article

Rapid detection of an Ebola biomarker with optical microring resonators

Journal

CELL REPORTS METHODS
Volume 2, Issue 6, Pages -

Publisher

CELL PRESS
DOI: 10.1016/j.crmeth.2022.100234

Keywords

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Funding

  1. T32 Cancer Biology Award [NIH CA009547]
  2. NIH [P01AI120943, R01AI123926, R41AI152745, R01AI141591, R01AI107056]
  3. Department of Pathology and Immunology PSTP program

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This study describes a rapid and sensitive diagnostic method for Ebola virus disease (EVD), using microring resonator sensors and the specific biomarker soluble glycoprotein (sGP) of Ebola infection. The method can detect sGP in a short time period with a low detection limit in serum.
Ebola virus (EBOV) is a highly infectious pathogen, with a casemortality rate as high as 89%. Rapid therapeutic treatments and supportive measures can drastically improve patient outcome; however, the symptoms of EBOV disease (EVD) lack specificity from other endemic diseases. Given the high mortality and significant symptom overlap, there is a critical need for sensitive, rapid diagnostics for EVD. Facile diagnosis of EVD remains a challenge. Here, we describe a rapid and sensitive diagnostic for EVD through microring resonator sensors in conjunction with a unique biomarker of EBOV infection, soluble glycoprotein (sGP). Microring resonator sensors detected sGP in under 40 min with a limit of detection (LOD) as low as 1.00 ng/mL in serum. Furthermore, we validated our assay with the detection of sGP in serum from EBOV-infected non-human primates. Our results demonstrate the utility of a high-sensitivity diagnostic platform for detection of sGP for diagnosis of EVD.

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