Journal
CELL REPORTS METHODS
Volume 2, Issue 6, Pages -Publisher
CELL PRESS
DOI: 10.1016/j.crmeth.2022.100224
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Funding
- SERB, DST [IPA/2020/000077]
- DBT [BT/PR30223/MED/2018]
- NII core grant
- NIH [75N9301900065, U01AI141995-03]
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The B cell help function of CD4(+) T cells is crucial in adaptive immunity. This study presents an improved method for quantitative measurement of T cell-dependent B cell responses using antigen-specific T-B cocultures, requiring as few as 90 T cells. The inclusion of monocytes in the cocultures enhances the interaction between T and B cells, leading to improved activation and immunological output.
The B cell help'' function of CD4(+) T cells is an important mechanism of adaptive immunity. Here, we describe improved antigen-specific T-B cocultures for quantitative measurement of T cell-dependent B cell responses, with as few as similar to 90 T cells. Utilizing M. tuberculosis (Mtb), we show that early priming and activation of CD4(+) T cells is important for productive interaction between T and B cells and that similar effects are achieved by supplementing cocultures with monocytes. We find that monocytes promote survivability of B cells via BAFF and stem cell growth factor (SCGF)/C-type lectin domain family 11 member A (CLEC11A), but this alone does not fully recapitulate the effects of monocyte supplementation. Importantly, we demonstrate improved activation and immunological output of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-specific memory CD4(+) T-B cell cocultures with the inclusion of monocytes. This method may therefore provide a more sensitive assay to evaluate the B cell help quality of memory CD4(+) T cells, for example, after vaccination or natural infection.
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