Journal
MEDICAL ONCOLOGY
Volume 40, Issue 1, Pages -Publisher
HUMANA PRESS INC
DOI: 10.1007/s12032-022-01893-8
Keywords
Crizotinib; Non-small cell lung cancer; Nanoparticles; Apoptosis; Liver toxicity
Categories
Funding
- Joint Funds for the innovation of science and Technology, Fujian province [2018Y9040]
- Fujian University of Traditional Chinese Medicine [X2021008]
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This study evaluated the effect and safety of crizotinib loaded polydopamine-polylactide-TPGS nanoparticles (CZT/pD-PT NPs) on non-small cell lung cancer (NSCLC). The results showed that CZT/pD-PT NPs had sustained-release effect and promoted the cellular uptake of CZT, leading to significant inhibition of NSCLC cell viability and promotion of cell apoptosis. Moreover, CZT/pD-PT NPs exhibited low hepatotoxicity. Therefore, CZT/pD-PT NPs may serve as a novel drug delivery system to improve the efficacy of chemotherapy for NSCLC.
To evaluate the effect and safety of crizotinib loaded polydopamine-polylactide-TPGS nanoparticles (CZT/pD-PT NPs) on non-small cell lung cancer (NSCLC). CZT/pD-PT NPs were synthesized and characterized, and their effects on PC-9 cell viability and apoptosis were determined. In vivo experiment was further performed to evaluate the anti-NSCLC efficacy of CZT/pD-PT NPs. TUNEL assay and Western blot were respectively applied for the determination of cell apoptosis and apoptosis-related protein expression, while liver function-related index expression detection and liver histopathological detection were used to evaluate the hepatotoxicity of CZT/pD-PT NPs. Compared with free CZT, CZT/pD-PT NPs had a sustained-release effect and promoted the cellular uptake of CZT. In addition, CZT/pD-PT NPs significantly inhibited PC-9 cell viability and promoted cell apoptosis both in vitro and in vivo, exhibiting superior cytotoxicity. At the same time, CZT/pD-PT NPs had no significant effect on liver tissue morphology and liver function-related indicators such as ALP, ALT, AST, and DBIL. CZT/pD-PT NPs have excellent anti-NSCLC effect with low hepatotoxicity, which can be served as a novel drug delivery system to improve the efficacy of chemotherapy for NSCLC.
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