Journal
SCIENCE SIGNALING
Volume 9, Issue 419, Pages -Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/scisignal.aad3895
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Funding
- DFG (Deutsche Forschungsgemeinschaft) [SFB1036, SFB938, SPP1710]
- BMBF (Federal Ministry of Education and Research) (LungSysII)
- Helmholtz Cross-Program topic Metabolic Dysfunction
- ICEMED (Imaging and Curing Environmental Metabolic Diseases) alliance
- DFG [EXC 1010, SFB-Tr 128, SPP 1710, EXC 114, SFB 870]
- European Research Council (ERC) [310932]
- European Research Council under the European Union [616791]
- German Cancer Research Center (DKFZ)
- Japan Society for the Promotion of Science
- Sao Paulo Research Foundation (FAPESP)
- DKFZ visiting scientist program
- Boehringer Ingelheim Fonds
- Medical Faculty, University of Heidelberg
- European Research Council (ERC) [616791, 310932] Funding Source: European Research Council (ERC)
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Mapping the in vivo distribution of endogenous oxidants in animal tissues is of substantial biomedical interest. Numerous health-related factors, including diet, physical activity, infection, aging, toxins, or pharmacological intervention, may cause redox changes. Tools are needed to pinpoint redox state changes to particular organs, tissues, cell types, and subcellular organelles. We describe a procedure that preserves the in vivo redox state of genetically encoded redox biosensors within histological tissue sections, thus providing redox maps for any tissue and comparison of interest. We demonstrate the utility of the technique by visualizing endogenous redox differences and changes in the context of tumor growth, inflammation, embryonic development, and nutrient starvation.
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