4.1 Article

Aberrant DNA methylation and expression of EYA4 in gastric cardia intestinal metaplasia

Journal

SAUDI JOURNAL OF GASTROENTEROLOGY
Volume 28, Issue 6, Pages 456-+

Publisher

WOLTERS KLUWER MEDKNOW PUBLICATIONS
DOI: 10.4103/sjg.sjg_228_22

Keywords

DNA methylation; EYA4; gastric cardia; intestinal metaplasia

Funding

  1. Project of Educational Commission of Guangdong Province of China [2017KQNCX068]
  2. Shantou Science and Technology Bureau [210712186880511, 220507236491772]
  3. Open Fund of Guangdong Provincial Key Laboratory of Infectious Diseases and Molecular Immunopathology [GDKL202209]
  4. Medical Scientific Research Foundation of Guangdong Province of China [A2022329]
  5. Huizhou Science and Technology Bureau [2021WC0106069]

Ask authors/readers for more resources

Gastric cardiac intestinal metaplasia (IM) is more common in older age and males. The development of gastric cardiac IM is associated with promoter hypermethylation and downregulation of the EYA4 gene.
Background: Intestinal metaplasia (IM) of the gastric cardia is an important premalignant lesion. However, there is limited information concerning its epidemiological and molecular features. Herein, we aimed to provide an overview of the epidemiological data for gastric cardiac IM and evaluate the role of EYA transcriptional coactivator and phosphatase 4 (EYA4) as an epigenetic biomarker for gastric cardiac IM. Methods: The study was conducted in the context of the gastric cardiac precancerous lesion program in southern China, which included 718 non-cancer participants, who undertook endoscopic biopsy and pathological examination in three endoscopy centers, between November 2018 and November 2021. Pyrosequencing and immunohistochemistry were performed to examine the DNA methylation status and protein expression level of EYA4. Results: Gastric cardiac IM presented in 14.1% (101/718) of participants and was more common among older (>50 years; 22.0% [95% CI: 17.8-26.8]) than younger participants (<= 50 years; 6.7% [95% CI: 4.5-9.9]; P < 0.001). IM was more common in male participants (16.9% [95% CI: 13.2-21.3] vs. 11.3% [95% CI: 8.3-15.1]; P = 0.04). Pyrosequencing revealed that IM tissues exhibited significantly higher DNA methylation levels in EYA4 gene than normal tissues (P = 0.016). Further, the protein expression level of EYA4 was reduced in IM and absent in intraepithelial neoplasia tissues compared to normal tissues (P < 0.001). Conclusions: Detection rates of gastric cardiac IM increase with age and are higher in men. Our findings highlight the important role of promoter hypermethylation and downregulation of EYA4 in gastric cardiac IM development.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.1
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available