Journal
NANOSCALE RESEARCH LETTERS
Volume 11, Issue -, Pages -Publisher
SPRINGER
DOI: 10.1186/s11671-016-1711-3
Keywords
3D Ag nanoflowers; Electrochemical immunosensor; E. coli; Bovine serum albumin
Funding
- National Natural Science Foundation of China [31400851, 81472001]
- Initial Foundation for Distinguished Scholars of Quanzhou Normal University
- Natural Science Foundation of Shanghai [15ZR1425300, 15JC1490600]
- SJTU [YG2014MS01]
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It is highly desirable to develop a rapid and simple method to detect pathogens. Combining nanomaterials with electrochemical techniques is an efficient way for pathogen detection. Herein, a novel 3D Ag nanoflower was prepared via a biomineralization method by using bovine serum albumin (BSA) as a template. It was adopted as a sensing interface to construct an electrochemical bacteria immunosensor for the rapid detection of foodborne pathogens Escherichia coli (E. coli) O157:H7. Bacterial antibody was immobilized onto the surface of Ag nanoflowers through covalent conjugation. Electrochemical impedance spectroscopy (EIS) was used to detect and validate the resistance changes, where [Fe(CN)(6)](3-/4-) acted as the redox probe. A linear relation between R-et and E. coli concentration was obtained in the E. coli concentration range of 3.0 x 10(2)-3.0 x 10(8) cfu mL(-1). The as-prepared biosensor gave rise to an obvious response to E. coli but had no distinct response to Cronobacter sakazakii, methicillin-resistant Staphylococcus aureus (MRSA), Staphylococcus albus, Lactobacillus easei, and Shigella flexneri, revealing a high selectivity for the detection of the pathogens down to 100 cfu mL(-1) in a short time. We believe that this BSA-conjugated 3D Ag nanoflowers could be used as a powerful interface material with good conductivity and biocompatibility for improving pathogen detection and treatment in the field of medicine, environment, and food safety.
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