Journal
ST PETERSBURG POLYTECHNIC UNIVERSITY JOURNAL-PHYSICS AND MATHEMATICS
Volume 15, Issue 3, Pages 207-212Publisher
POLYTECHNICAL UNIV PUBLISHING HOUSE
DOI: 10.18721/JPM.153.340
Keywords
polymerase chain reaction (PCR); microfluidic chip; DNA; fluorescence; dyes; thermal cycler; optical fiber; amplification
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In this study, the creation and testing of a prototype for recording fluorescence from microfluidic chips during the polymerase chain reaction (PCR) is considered. The paper presents the characteristics of the main elements used to create the layout of the fluorescence detection device. Results from experiments testing the performance of mock-up elements and microfluidic chips are presented. The operability of the assembled layout was demonstrated during real-time PCR reaction.
In this paper, we consider the creation and testing of a prototype for recording fluorescence from microfluidic chips during the polymerase chain reaction (PCR). The paper presents the characteristics of the main elements used to create the layout of the device for fluorescence detection. The results of experiments in testing the performance of mock-up elements and microfluidic chips are presented. The operability of the assembled layout was demonstrated during the real-time PCR reaction.
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