Journal
MEDICAL SCIENCE MONITOR
Volume 22, Issue -, Pages 3562-3575Publisher
INT SCIENTIFIC INFORMATION, INC
DOI: 10.12659/MSM.897773
Keywords
Angiogenesis Inhibitors; Apoptosis; Cell Proliferation; Myocardial Infarction; PTEN Phosphohydrolase; Vascular Endothelial Growth Factor; Endocrine-Gland-Derived
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Background: This study investigated how miR-21 expression is reflected in acute myocardial infarction and explored the role of miR-21 and the PTEN/VEGF signaling pathway in cardiac microvascular endothelial cells. Material/Methods: We used an in vivo LAD rat model to simulate acute myocardial infarction. MiR-21 mimics and miR-21 inhibitors were injected and transfected into model rats in order to alter miR-21 expression. Cardiac functions were evaluated using echocardiographic measurement, ELISA, and Masson staining. In addition, lenti-PTEN and VEGF siRNA were transfected into CMEC cells using standard procedures for assessing the effect of PTEN and VEGE on cell proliferation, apoptosis, and angiogenesis. MiR-21, PTEN, and VEGF expressions were examined by RTPCR and Western blot. The relationship between miR-21 and PTEN was determined by the luciferase activity assay. Results: We demonstrated that miR-21 bonded with the 3'-UTR of PTEN and suppressed PTEN expressions. Established models significantly induced cardiac infarct volume and endothelial injury marker expressions as well as miR21 and PTEN expressions (P< 0.05). MiR-21 mimics exhibited significantly protective effects since they down-regulated both infarction size and injury marker expressions by increasing VEGF expression and inhibiting PTEN expression (P< 0.05). In addition, results from in vitro research show that lenti-PTEN and VEGF siRNA can notably antagonize the effect of miR-21 on cell proliferation, apoptosis, and angiogenesis (P< 0.05). Conclusions: MiR-21 exerts protective effects on endothelial injury through the PTEN/VEGF pathway after acute myocardial infarction.
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