Journal
POSTEPY W KARDIOLOGII INTERWENCYJNEJ
Volume 18, Issue 4, Pages 392-398Publisher
TERMEDIA PUBLISHING HOUSE LTD
DOI: 10.5114/aic.2022.120994
Keywords
diffusion; yeast cells; low-field NMR; D-T2; T1-T2
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Funding
- National Centre for Research and Development [STRATE- GMED2/265761/10/NCBR/2015, PBS2/A2/16/2013]
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In this study, model cell suspensions were characterized using low-field 1H NMR relaxometry. A multi-parametric characterization based on independent 2D T1-T2 and D-T2 measurements was implemented to obtain a set of MR parameters as a specific signature for model cells.
Introduction: Nuclear magnetic resonance (NMR) is a powerful method for the non-invasive study of a wide range of objects. Among its many characteristics, molecular diffusion can be examined without the need for any chemical or isotopic tracers by ap-plying magnetic field gradients within the NMR sequence.Aim: In our study, model cell suspensions were characterized by means of low-field (LF) (0.05 T) 1H NMR relaxometry. The pro-posed multi-parametric characterization based on independent 2D T1-T2 and D-T2 measurements was implemented to obtain a set of MR parameters as a specific signature for model cells.Material and methods: The D-T2 and T1-T2 correlation measurements were conducted on yeast samples with different amounts of added water. Signals from intracellular and extracellular water compartments and free water were identified on D-T2 maps and their diffusion coefficients were extracted.Results: Mean D_IC was equal to 8.4 x 10-11 m2/s and mean D_EC ranged from 1.0 x 10-9 m2/s to 1.65 x 10-9 m2/s. T1/T2 ratio was calculated and for IC space values in the range of 4.2-5.3 were observed. Finally, we demonstrated the possibility of detecting signals from cells for the samples with a low concentration of cell suspensions or a small amount of the sample.Conclusions: These findings are promising for more complex cell investigations in vitro and in vivo, without any contrast agents, applying solely biomarkers.
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