Genetic and molecular analysis of starch physicochemical properties and its relationship with endosperm modification in quality protein maize

Quality protein maize (QPM) was created converting the soft opaque-2 endosperm into a vitreous phenotype, but the mechanisms involved in this modification are not completely understood. Recombinant inbred lines (RILs) derived from K0326Y QPM and W64Ao2 were used to identify quantitative trait loci (QTL) associated with starch physicochemical properties. Vitreousness-contrasting RILs were also used to evaluate the expression of starch biosynthesis genes. Mapping identified 5-6 QTLs for each trait that explained 66 % of the phenotypic variation. Three QTLs on bins 4.05, 5.04, and 9.03 were found close to the starch biosynthesis genes Brittle-2 (Bt2), Amylose extender-1 (Ae1), and Waxy-1 (Wx1), respectively. The expression of Wx1 was three-fold greater in K0326Y QPM than W64Ao2, and eight-fold higher in vitreous than opaque RILs, which corresponded with the greater granule bound starch synthase I (GBSSI) accumulation and the higher amylose content observed in the vitreous lines. The increased synthesis of amylose may result in starch granules with more amorphous regions that favor their compaction. These results suggest that endosperm modification in QPM is associated with the synthesis of starch containing more amylose during kernel development, which may facilitate the packing of the starch granules resulting in the vitreous phenotype.

Automated summary

This study identified quantitative trait loci (QTL) associated with starch physicochemical properties in quality protein maize (QPM) using recombinant inbred lines (RILs). The research results suggest that the expression differences of starch biosynthesis genes and starch components may contribute to the formation of the vitreous phenotype in QPM maize.