4.3 Article

Ajania purpurea Extract Attenuates LPS-Induced Inflammation in RAW264.7 Cells and Peritonitis Mice

Journal

BIOLOGICAL & PHARMACEUTICAL BULLETIN
Volume 45, Issue 12, Pages 1847-1852

Publisher

PHARMACEUTICAL SOC JAPAN

Keywords

Ajania purpurea; inflammation; nuclear factor-kappaB (NF-kappa B); RAW264.7 cell; mouse

Funding

  1. National Natural Science Foundation of China [82074578, 81960775, 81960781]
  2. Shandong Province Natural Science Fundation [ZR2021LZY032]

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Ajania purpurea Shih. ethanol extract (EAPS) suppresses inflammation by inhibiting the NF-kappa B pathway in RAW264.7 cells and mice with peritonitis, reducing the release of inflammatory factors and recruitment of immune cells.
Macrophages have important roles in the progression of inflammation. Ajania purpurea Shih. is a member of the Ajania Poljakor family that grows in Tibet (China). Extracts from plants in this genus have antibacterial and anti-inflammatory properties. However, there are few reports on the activity and mechanism of Ajania purpurea. Here, we confirmed the anti- inflammatory effect of Ajania purpurea Shih. ethanol extract (EAPS) by examining the levels of inflammatory factors in a mouse model of peritonitis and RAW264.7 cells. The main components of EAPS detected by LC-MS analysis included piperine and chlorogenic acid. In particular, in lipopolysaccharide (LPS)-induced RAW264.7 cells, EAPS inhibited the protein expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in LPS-induced RAW264.7 cells, lowered the levels of nitric oxide ( NO) and prostaglandin E2 (PGE(2)), as well as the release of inflammatory factors such as tumor necrosis factor-alpha (TNF-alpha) and pro-inflammatory cytokines such as interleukin (IL)-1 beta and IL-6. In addition, Western blot analysis and immunofluorescence staining verified that EAPS inhibited the activity of the nuclear factor-kappaB (NF-kappa B) pathway by reducing the nuclear translocation of the p65 subunit. Furthermore, in a mouse model of peritonitis, EAPS inhibited the release of inflammatory factors, as well as the recruitment of immune cells including neutrophils and macrophages. These findings indicated that EAPS suppressed LPS-induced inflammation via inhibiting the NF-kappa B pathway in RAW264.7 cells and mice with peritonitis. Thus, EAPS may be a viable therapeutic method for the treatment of inflammation and related disorders.

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