Journal
STAR PROTOCOLS
Volume 3, Issue 4, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.xpro.2022.101719
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Funding
- T32 Cancer Biology Award [P01AI120943]
- NIH [R01AI123926, R41AI152745, R01AI141591, R01AI107056]
- Department of Pathology and Immunology PSTP program
- [NIH CA009547]
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This article describes the use of silicon photonic microring resonator sensors for detecting Ebola virus and Sudan virus soluble glycoprotein. The protocol includes chip functionalization, protein detection in sera, calibration standard preparation, and quantification of results. This method can be adapted for other analytes.
This protocol describes the use of silicon photonic microring resonator sensors for detection of Ebola virus (EBOV) and Sudan virus (SUDV) soluble glycoprotein (sGP). This protocol encompasses biosensor functionalization of silicon microring resonator chips, detection of protein biomarkers in sera, preparing calibration standards for analytical validation, and quantification of the results from these experiments. This protocol is readily adaptable toward other analytes, including cytokines, chemokines, nucleic acids, and viruses. For complete details on the use and execution of this protocol, please refer to Qavi et al. (2022).
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