Preparation of a murine oral palate wound healing model

The oral cavity is a highly regenerative epithelial tissue that results in minimal scarring after injury. This protocol describes the preparation of a mouse palate wound model. The protocol includes steps to place an excisional wound on the mouse palate, followed by harvesting of wound tissue and bone decalcification. We detail how to overcome the technical challenge of limited anatomical space, avoid damaging the nasal cavity, manage bleeding, and collect tissue for down-stream genomic or immunohistochemical analysis.

Automated summary

This protocol provides a detailed guide on how to prepare a mouse palate wound model, including techniques to overcome the challenge of limited anatomical space, avoid damaging the nasal cavity, control bleeding, and collect tissue for downstream analysis.