Journal
BIOACTIVE MATERIALS
Volume 19, Issue -, Pages 581-593Publisher
KEAI PUBLISHING LTD
DOI: 10.1016/j.bioactmat.2024.04.025
Keywords
Decellularized matrix; Annulus fibrosus; Hydrogel; THF-beta 1; Tissue repair
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This study developed an injectable hydrogel for annulus fibrosus (AF) repair by combining polyethylene glycol diacrylate (PEGDA) and decellularized annulus fibrosus matrix (DAFM). Transforming growth factor-beta 1 (TGF-beta 1) was also incorporated into the hydrogel. The hydrogel showed improved mechanical strength, facilitated AF cell migration, adhesion, proliferation, and increased extracellular matrix (ECM) production. In vivo experiments showed that the hydrogel effectively repaired AF defects and restored biomechanical properties of the disc.
Annulus fibrosus (AF) repair remains a challenge because of its limited self-healing ability. Endogenous repair strategies combining scaffolds and growth factors show great promise in AF repair. Although the unique and beneficial characteristics of decellularized extracellular matrix (ECM) in tissue repair have been demonstrated, the poor mechanical property of ECM hydrogels largely hinders their applications in tissue regeneration. In the present study, we combined polyethylene glycol diacrylate (PEGDA) and decellularized annulus fibrosus matrix (DAFM) to develop an injectable, photocurable hydrogel for AF repair. We found that the addition of PEGDA markedly improved the mechanical strength of DAFM hydrogels while maintaining their porous structure. Transforming growth factor-beta 1 (TGF-beta 1) was further incorporated into PEGDA/DAFM hydrogels, and it could be continuously released from the hydrogel. The in vitro experiments showed that TGF-beta 1 facilitated the migration of AF cells. Furthermore, PEGDA/DAFM/TGF-beta 1 hydrogels supported the adhesion, proliferation, and increased ECM production of AF cells. In vivo repair performance of the hydrogels was assessed using a rat AF defect model. The results showed that the implantation of PEGDA/DAFM/TGF-beta 1 hydrogels effectively sealed the AF defect, prevented nucleus pulposus atrophy, retained disc height, and partially restored the biomechanical properties of disc. In addition, the implanted hydrogel was infiltrated by cells resembling AF cells and well integrated with adjacent AF tissue. In summary, findings from this study indicate that TGF-beta 1-supplemented DAFM hydrogels hold promise for AF repair.
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