Journal
JOURNAL OF THE AMERICAN HEART ASSOCIATION
Volume 5, Issue 9, Pages -Publisher
WILEY
DOI: 10.1161/JAHA.116.003675
Keywords
Na/K-ATPase; protein carbonylation; protein trafficking; reactive oxygen species; signaling; sodium transport
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Funding
- NIH [RO1 HL-109015, HL071556, RO1 HL-105649]
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Background-We have demonstrated that cardiotonic steroids, such as ouabain, signaling through the Na/K-ATPase, regulate sodium reabsorption in the renal proximal tubule. By direct carbonylation modification of the Pro222 residue in the actuator (A) domain of pig Na/K-ATPase alpha 1 subunit, reactive oxygen species are required for ouabain-stimulated Na/K-ATPase/c-Src signaling and subsequent regulation of active transepithelial Na-22(+) transport. In the present study we sought to determine the functional role of Pro222 carbonylation in Na/K-ATPase signaling and sodium handling. Methods and Results-Stable pig alpha 1 knockdown LLC-PK1-originated PY-17 cells were rescued by expressing wild-type rat alpha 1 and rat alpha 1 with a single mutation of Pro224 (corresponding to pig Pro222) to Ala. This mutation does not affect ouabain-induced inhibition of Na/K-ATPase activity, but abolishes the effects of ouabain on Na/K-ATPase/c-Src signaling, protein carbonylation, Na/K-ATPase endocytosis, and active transepithelial Na-22(+) transport. Conclusions-Direct carbonylation modification of Pro224 in the rat alpha 1 subunit determines ouabain-mediated Na/K-ATPase signal transduction and subsequent regulation of renal proximal tubule sodium transport.
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