Sensitive detection of aflatoxin B1 in foods by aptasensing-based qPCR

Article Chemistry, Applied

A stem-loop like aptasensor for sensitive detection of aflatoxin based on graphene oxide/AuNPs nanocomposite platform

Mehdi Dadmehr et al.

Summary: A specific aptasensor for aflatoxin B1 (AFB1) detection was developed in this study, which showed high sensitivity and selectivity for detecting AFB1 in food samples.

FOOD CHEMISTRY (2023)

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Article Chemistry, Analytical

Microelectromechanical Microsystems-Supported Photothermal Immunoassay for Point-of-Care Testing of Aflatoxin B1 in Foodstuff

Zhichao Yu et al.

Summary: A portable photothermal immunosensing platform supported by a microelectromechanical microsystem (MEMS) was developed for quick and cost-effective identification of acutely toxic and low-fatality mycotoxins, which is critical for reducing population mortality.

ANALYTICAL CHEMISTRY (2023)

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Article Biochemical Research Methods

Simultaneous Dual-Sensing Platform Based on Aptamer-Functionalized DNA Hydrogels for Visual and Fluorescence Detection of Chloramphenicol and Aflatoxin M1

Gulnur Camizci Aran et al.

Summary: A chloramphenicol and aflatoxin M1 aptamer-functionalized DNA hydrogel was designed for the simultaneous detection of chloramphenicol and aflatoxin M1. By using acrydite-modified chloramphenicol aptamer sequence, the DNA hydrogel was synthesized and used for visual detection of chloramphenicol. The DNA hydrogel was formulated with 60% linear polyacrylamide-DNA conjugate, 40% acrylamide and chloramphenicol aptamer/DNA strand-1 at a molar ratio of 1:1, with the lowest concentration of chloramphenicol for gel dissociation being 1.0 nM at 25 degrees C. The formalized aptamer-functionalized DNA hydrogel was also used to detect aflatoxin M1 by measuring the recovery of fluorescence signal. The detection platform showed promising results for the simultaneous detection of chloramphenicol and aflatoxin M1 in aqueous solution and milk.

BIOCONJUGATE CHEMISTRY (2023)

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Article Environmental Sciences

Aflatoxin B1 exposure induced developmental toxicity and inhibited muscle development in zebrafish embryos and larvae

Xiang-Ning He et al.

Summary: The prevalence of Aflatoxin B1 (AFB1), a highly toxic mycotoxin, is increasing worldwide and poses various health problems. The direct toxicity of AFB1 on embryonic development, especially muscle development, has not been extensively studied. This study used zebrafish embryos as a model to investigate the mechanism of AFB1's direct toxicity on embryonic development, revealing inhibition of muscle development through oxidative damage, apoptosis, and disruption of tight junction complexes (TJs).

SCIENCE OF THE TOTAL ENVIRONMENT (2023)

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Article Biochemistry & Molecular Biology

Total Flavonoids of Rhizoma Drynariae Mitigates Aflatoxin B1-Induced Liver Toxicity in Chickens via Microbiota-Gut-Liver Axis Interaction Mechanisms

Shucheng Huang et al.

Summary: This study investigated the protective effects and potential mechanisms of total flavonoids of Rhizoma Drynaria (TFRD), a traditional Chinese medicinal herb, against Aflatoxin B1 (AFB1)-induced liver injury. The results showed that TFRD supplementation improved intestinal permeability, corrected changes in gut microbiota and liver damage, and improved plasma metabolites affected by AFB1 exposure. Furthermore, TFRD treatment suppressed oxidative stress, hepatic lipid deposition, and hepatic ferroptosis gene expression. These findings suggest that TFRD can potentially counteract the detrimental effects of mycotoxins.

ANTIOXIDANTS (2023)

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Article Chemistry, Applied

AFB1 colorimetric aptamer sensor for the detection of AFB1 in ten different kinds of miscellaneous beans based on gold nanoparticles and smartphone imaging

Lifeng Lu et al.

Summary: A simple and low-cost smartphone-assisted assay was developed for measuring aflatoxin B1 in miscellaneous beans. The assay utilized a modified QuEChERS method with aptamers and gold nanoparticles as recognition elements and indicators, respectively. The assay showed good sensitivity and specificity, with a linear range of 0.2-8.0 ng/g and a detection limit of 0.08 ng/g. The recovery rate of aflatoxin B1 in bean samples ranged from 87.18% to 110.24%. The entire process took only 15 minutes. This colorimetric aptamer sensor can be used for quick aflatoxin B1 detection in beans.

FOOD CHEMISTRY (2023)

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Article Chemistry, Analytical

Comparison of lateral flow immunoassays based on oriented and nonoriented immobilization of antibodies for the detection of aflatoxin B1

Xinyi Mao et al.

Summary: Studies have shown that oriented immobilization of antibodies improves sensitivity in sandwich immunoassays for protein detection, but has limited effect on competitive immunoassays for small molecule detection. However, this method can enhance detection precision and matrix tolerance.

ANALYTICA CHIMICA ACTA (2022)

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Article Chemistry, Analytical

On-bead DNA synthesis triggered by allosteric probe for detection of carcinoembryonic antigen

Min Ling et al.

Summary: This article introduces an on-bead DNA synthesis system for amplifying protein signals, which can sensitively quantify protein biomarkers and has great potential for clinical applications.

MICROCHIMICA ACTA (2022)

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Article Chemistry, Analytical

Highly sensitive SERS detection for Aflatoxin B1 and Ochratoxin A based on aptamer-functionalized photonic crystal microsphere array

Laicui Song et al.

Summary: A highly sensitive surface-enhanced Raman spectroscopy (SERS) aptasensor for multiplex mycotoxins detection was developed based on aptamer-functionalized photonic microsphere array. This method has the potential for high sensitivity, low-cost, and high throughput.

SENSORS AND ACTUATORS B-CHEMICAL (2022)

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Article Nutrition & Dietetics

Chronic aflatoxin exposure during pregnancy is associated with lower fetal growth trajectories: a prospective cohort from the Butajira Nutrition, Mental Health, and Pregnancy (BUNMAP) Study in rural Ethiopia

Kokeb Tesfamariam et al.

Summary: This study examined the association between chronic aflatoxin exposure during pregnancy and fetal growth trajectories in a rural setting in Ethiopia. The findings suggest that maternal aflatoxin exposure is associated with lower fetal growth over time.

AMERICAN JOURNAL OF CLINICAL NUTRITION (2022)

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Article Chemistry, Applied

Simultaneous determination of aflatoxins B1, B2, G1 and G2 in commercial rices using immunoaffinity column clean-up and HPLC-MS/MS

Ivan Romero-sanchez et al.

Summary: This study evaluated different methods for the extraction and clean-up of aflatoxins from rice, and successfully applied the method to analyze rice samples from Spanish supermarkets. The results showed that the method of methanol-water extraction combined with immunoaffinity column clean-up had favorable performance, with high recoveries and low detection and quantification limits.

FOOD CHEMISTRY (2022)

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Article Chemistry, Applied

Surface-anchored liquid crystal droplets for the semi-quantitative detection of Aflatoxin B1 in food samples

Supan Cheng et al.

Summary: In this study, a liquid-crystal (LC) based assay for rapid detection of AFB1 in food samples was designed. The LC-based sensing platform provides simple operation, remarkable sensitivity, high selectivity, low cost, and excellent portability without the use of any bulky instrument, which is very promising in rapid on-field detection of mycotoxins.

FOOD CHEMISTRY (2022)

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Article Food Science & Technology

Determining aflatoxins in raw peanuts using immunoaffinity column as sample clean-up method followed by normal-phase HPLC-FLD analysis

Ming-Hsun Shen et al.

Summary: This study aimed to simplify the conventional aflatoxin analysis method by utilizing the immunoaffinity column and normal-phase chromatography to detect aflatoxins B1, B2, G1, and G2. The optimized mobile phase enhanced fluorescence signal and improved sample cleanup method significantly increased recovery and repeatability. The proposed method outperformed the official method and met the US FDA's standards for examining human food, with a prolonged shelf-life of the mobile phase under room temperature.

FOOD CONTROL (2022)

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Article Engineering, Environmental

Integrating magnetic metal-organic frameworks-based sample preparation with microchannel resistance biosensor for rapid and quantitative detection of aflatoxin B1

Xiaohan Li et al.

Summary: An integrated detection strategy combining Mg/Zn-MOF-74 modified Fe3O4 magnetic nanoparticles-based sample preparation and microchannel resistance biosensor was developed for rapid and highly sensitive detection of aflatoxin B-1 in food samples.

JOURNAL OF HAZARDOUS MATERIALS (2022)

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Article Chemistry, Multidisciplinary

Rapid and label-free detection of aflatoxin B1 using a rationally truncated aptamer and via circular dichroism measurement

Chao Wang et al.

Summary: We present a simple aptamer assay for rapid analysis of the carcinogenic fungal metabolite AFB1. By rationally truncating the original anti-AFB1 aptamer, a short aptamer with improved performance was obtained. Different concentrations of AFB1 were detected by measuring the circular dichroism spectra of this short aptamer. The detection limit reached 0.6 nM, which was lower than that of the original aptamer (80 nM).

CHEMICAL COMMUNICATIONS (2022)

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Article Chemistry, Analytical