Vibrational spectroscopy methods for investigation of the animal models of glioblastoma multiforme

Glioblastoma multiforme (GBM) is the most common and devastating primary brain tumor among adults. It is highly lethal disease, as only 25% of patients survive longer than 1 year and only 5% more than 5 years from the diagnosis. To search for the new, more effective methods of treatment, the understanding of mechanisms underlying the process of tumorigenesis is needed.The new light on this problem may be shed by the analysis of biochemical anomalies of tissues affected by tumor growth. Therefore, in the present work, we applied the Fourier transform infrared (FTIR) and Raman microspectroscopy to evaluate changes in the distribution and structure of biomolecules appearing in the rat brain as a result of glioblastoma development. In turn, synchrotron X-ray fluorescence microscopy was utilized to determine the elemental anomalies appearing in the nervous tissue. To achieve the assumed goals of the study animal models of GBM were used. The rats were subjected to the intracranial implantation of glioma cells with different degree of invasiveness. For spectroscopic investigation brain slices taken from the area of cancer cells administration were used.

Automated summary

Glioblastoma multiforme (GBM) is a common and deadly brain tumor in adults. This study utilized Fourier transform infrared (FTIR) and Raman microspectroscopy to evaluate changes in biomolecular distribution and structure caused by glioblastoma development in rat brains, and used synchrotron X-ray fluorescence microscopy to determine elemental anomalies in nervous tissue.