Synthesis of NMN by cascade catalysis of intracellular multiple enzymes

& beta;-Nicotinamide mononucleotide is a biologically active nucleotide compound, and its excellent anti-aging activity is widely used in medicine and has multiple functions, making NMN have broad application prospects in the fields of nutrition, health food, and even medicine. Herein, based on the supply of the co-substrate PRPP, we designed and constructed three in vivo NMN synthesis pathways using glucose, xylose, and arabinose as raw materials and Escherichia coli as the host. The best in vivo pathway through whole-cell catalysis was identified. Then, we optimized the cell culture and catalytic conditions of the optimal path to determine the optimal conditions and ultimately obtained an NMN titer of 1.8 mM. Three multi-enzyme cascade catalytic pathways were constructed to explore the supply capacity of glucose, xylose, and arabinose to PRPP. 1.8 mM NMN was ultimately produced under optimized conditions using glucose as the substrate.

Automated summary

This study constructed three in vivo NMN synthesis pathways based on the supply of co-substrate PRPP. By optimizing the conditions, a yield of 1.8 mM NMN was obtained. The research provides important theoretical and practical evidence for the application of NMN in the field of anti-aging.