Journal
JOURNAL OF COLLOID AND INTERFACE SCIENCE
Volume 653, Issue -, Pages 351-358Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jcis.2023.09.067
Keywords
Protein corona; Super -resolution imaging; PEGylation; Penetration depth; Visualization; Mesoporous silica particle
Categories
Ask authors/readers for more resources
This study demonstrates the direct observation and quantification of protein corona adsorbed onto PEGylated mesoporous silica particles using direct stochastic optical reconstruction microscopy. The effects of PEG molecular weights and incubation time on protein penetration depth are investigated for the first time.
Nanoparticles (NPs) adsorb serum proteins when exposed to biological fluids, forming a dynamic protein corona that has a profound impact on their overall biological profile and fate. Polyethylene glycol (PEG) modification is the most widely used strategy to mitigate and inhibit protein corona formation. Nevertheless, the accurate mapping and quantification of PEG inhibition effects on protein corona formation have scarcely been reported. Herein, we demonstrate the direct observation and quantification of protein corona adsorbed onto PEGylated mesoporous silica particles by direct stochastic optical reconstruction microscopy (dSTORM). The variation tendency of protein penetration depth in terms of PEG molecular weights and incubated time is investigated for the first time. The maximum penetration depths present slight increase with the prolonged incubation time, while they tend to remarkably decrease with increased chain length of modified PEG. Moreover, the colocalization of preformed protein corona with lysosomes and the destination of adsorbed protein are demonstrated. Our method provides important technical characterization information and in-depth understanding of
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available