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ANALYTICAL CHEMISTRY
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Penghui Liang et al.
Summary: This paper presents a dual-mode lateral flow immunoassay (LFIA) based on Ag-MBA@Au nanoparticles for colorimetric and SERS detection of SARS-CoV-2 IgG. The method shows high sensitivity, good reproducibility, and specificity, and demonstrates excellent consistency in clinical serum sample testing.
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Hyun-Kyung Oh et al.
Summary: This study developed AuNP clusters to improve the detection sensitivity of lateral flow immunoassays (LFI) for diseases, including SARS-CoV-2. The AuNP clusters maintained the red color of isolated individual AuNPs while increasing the colorimetric readout. The PLASCOP AuNP clusters showed high sensitivity and good specificity for SARS-CoV-2 nucleocapsid proteins, making them a promising sensing tool for LFI applications.
BIOSENSORS & BIOELECTRONICS
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Immunology
Amresh Kumar et al.
Summary: The nucleoprotein of SARS-CoV-2 has been shown to elicit a cell-mediated immune response, regulating viral replication and reducing viral burden. However, the development of an effective vaccine against SARS-CoV-2 remains a challenge. In this study, researchers focused on a chimeric protein vaccine composed of the nucleoprotein and S-1 protein components of SARS-CoV-2, aiming to induce a strong cellular immune response and neutralize different variants of the virus while limiting its attachment, replication, and release in host cells.
EXPERT REVIEW OF VACCINES
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Review
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Qing Ye et al.
Summary: SARS-CoV-2 antigen detection is a supplementary method for COVID-19 diagnosis, which can meet clinical needs to some extent, but it has low sensitivity for asymptomatic individuals in low prevalence areas, leading to missed diagnoses, and there are differences in detection ability for different virus strains. Although the SARS-CoV-2 antigen assay has high reliability in excluding infection, it has a high false positive rate in low prevalence environments and needs to be confirmed by nucleic acid detection.
JOURNAL OF MEDICAL VIROLOGY
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Yuen Yung Hui et al.
Summary: In this study, a low-cost and portable lateral flow immunoassay (LFIA) reader based on photothermal detection was developed. The LFIA reader utilized a low-power green laser as the heating source and a single-element infrared sensor as the detector for accurate quantitative detection of reporters captured on LFIA strips. The results showed that photothermal detection significantly enhanced the sensitivity of LFIA and enabled quantitative analysis to support critical decision-making.
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(2022)
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Multidisciplinary Sciences
Terumitsu Azuma et al.
Summary: Temperature sensing is a promising method for enhancing the detection sensitivity of lateral flow immunoassay (LFIA) in point-of-care testing. By exciting fluorescent particles or colored latex beads, a temperature increase of over 100 degrees C can be achieved with a low-power laser. This study provides a fundamental understanding of thermometric assay using non-fluorescent latex beads deposited on a nitrocellulose membrane. The research reveals that the absorption of light by red and black latex beads at 520 nm is enhanced by 1.3-fold and 3.2-fold, respectively, due to the multiple scattering of light in the highly porous medium. The CLB-based thermometric assay demonstrates a 10x higher sensitivity than color visualization, similar to previous studies using gold nanoparticles. The practical application of this thermometric immunoassay for rapid antigen tests of COVID-19 is demonstrated.
SCIENTIFIC REPORTS
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Nol Salcedo et al.
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Rowa Y. Alhabbab et al.
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Review
Biochemical Research Methods
Soohyun Kim et al.
Summary: This article introduces the viral structure and diagnostic biomarkers of the new coronavirus, and discusses the limitations and unresolved issues of paper-based diagnostic platforms. In addition, it provides insights into the prospects of paper-based diagnostic platforms integrated with advanced technologies such as nanotechnology, aptamers, SERS, and CRISPR-Cas.
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Engineering, Biomedical
Lun Bian et al.
Summary: The researchers developed an ultrabright nanoparticles-based lateral flow immunoassay for rapid semi-quantitative detection of anti-SARS-CoV-2 neutralizing antibodies in serum samples. The method accurately identified negative and positive samples with high precision. The study highlights the practical value of the established assay in assessing acquired protective immunity against SARS-CoV-2.
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Biophysics
Xingyu Ye et al.
Summary: In this study, a multiplex nucleic acid detection system called MULAN was developed, which integrates rapid isothermal amplification with the multiplex inclusiveness of a single Ago for simultaneous detection of multiple viruses. MULAN showed high specificity and sensitivity, and could be used for portable and visible detection.
BIOSENSORS & BIOELECTRONICS
(2022)
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Medical Laboratory Technology
Chang Chu et al.
Summary: Both infection with and vaccination against SARS-CoV-2 can trigger a complex B-cell and T-cell response. Reliable methods for analyzing the B-cell response are well established, but methods for measuring the T-cell response still need to be proven in clinical settings. In this study, a T-cell proliferation assay based on 3H thymidine incorporation was developed and validated. The assay uses SARS-CoV-2 derived peptide pools to stimulate T-cell responses. The novel SARS-CoV-2 lymphocyte transformation test (SARS-CoV-2 LTT) showed excellent discrimination of infected and/or vaccinated individuals versus unexposed controls, with an area under the curve (AUC) of >0.95 in ROC analysis. Furthermore, the assay can also differentiate T-cell responses resulting from vaccination against those induced by infection by using peptide pools representing not only the S protein but also the N and M proteins.
CLINICA CHIMICA ACTA
(2022)
Article
Infectious Diseases
Jinfeng Li et al.
Summary: A quantum dot lateral flow immunoassay strip (QD-LFIA) for smartphones was designed to detect specific IgG or neutralizing antibodies in SARS-CoV-2. The results showed high sensitivity and specificity in detecting antibodies in recovered patients and vaccinated individuals, with no cross-reaction observed.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2022)
Review
Immunology
Mingna Li et al.
Summary: The outbreak of COVID-19 and the increasing virulence of mutant strains have posed a tremendous threat to global public health. LAMP technology, with its advantages of convenience, speed, low cost, and high sensitivity, plays an important role in the detection of SARS-CoV-2.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
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Medical Laboratory Technology
Buzhou Xu et al.
Summary: The study developed a rapid, sensitive, and specific SARS-CoV-2 detection method that can complete sample testing within 15 minutes, showing high sensitivity, specificity, and accuracy in clinical trials.
JOURNAL OF CLINICAL LABORATORY ANALYSIS
(2022)
Review
Biochemistry & Molecular Biology
Shuyuan Qiao et al.
Summary: This review summarizes the structural basis of S-mediated cell entry of SARS-CoV, MERS-CoV and SARS-CoV-2, and discusses the evolution of the S glycoprotein for cross-species transmission. It also describes the recent progress in developing therapeutic methods targeting the SARS-CoV-2 S glycoprotein.
Review
Medicine, General & Internal
Wenxia Shao
Summary: SARS-CoV-2 is a serious respiratory virus that can cause severe damage and even death. Early diagnosis is crucial, and the current gold standard is nucleic acid detection. However, this method has limitations in terms of requirements and time-consuming process. Antigen tests, while not diagnostic, have potential for early triage. Improvements in detection technology and reagents are urgently needed to overcome current limitations.
FRONTIERS IN MEDICINE
(2022)
Review
Chemistry, Analytical
Sushma Thapa et al.
Summary: This article outlines the devastating impact of the global COVID-19 pandemic on human health. It also explores traditional tools for diagnosing SARS-CoV-2 and the role of emerging nanomaterials in creating biosensor devices for diagnosis.
Article
Chemistry, Analytical
Pearl Thompson et al.
Summary: A rapid lateral flow screening tool using saliva samples and cell phone quantification has been developed, providing sensitive detection of IgG antibodies against SARS-CoV-2 within 10 minutes.
Article
Nanoscience & Nanotechnology
Donggu Hong et al.
Summary: A novel rapid detection method using gold nanoparticle assembled SiO2 core-satellite nanoparticles has been developed for sensitive detection of SARS-CoV-2. The method achieves highly sensitive detection within 20 minutes and significantly improves the detection sensitivity by utilizing wellgrown AuNP satellites.
ACS APPLIED MATERIALS & INTERFACES
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Chemistry, Analytical
Wesley Wei -Wen Hsiao et al.
Summary: This report presents a novel detection platform called SELFIA for rapid testing of different variants of SARS-CoV-2 virus. The platform shows comparable accuracy and sensitivity with traditional testing methods.
ANALYTICA CHIMICA ACTA
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Article
Chemistry, Analytical
Zihao Xie et al.
Summary: A novel magnetic/fluorescent dual-modal lateral flow immunoassay has been developed for the rapid and accurate diagnosis of SARS-CoV-2 nucleocapsid protein. This method combines the advantages of magnetic and fluorescent signals, resulting in high sensitivity and accuracy, and shows potential for early-stage diagnosis.
ANALYTICA CHIMICA ACTA
(2022)
Article
Biophysics
Cheonjung Kim et al.
Summary: A nanoelectrokinetic-assisted lateral flow assay platform is proposed to enhance the sensitivity and limit of detection for self-testing, especially in the context of the COVID-19 pandemic, by using a microfluidic paper-based preconcentrator. This preconcentration technique improved the performance of the lateral flow assay by 32-fold and increased the analytical sensitivity by 16.4%, offering a new opportunity for point-of-care testing and self-testing.
BIOSENSORS & BIOELECTRONICS
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Article
Biophysics
Haoyang Tong et al.
Summary: An AI-assisted PDA-based LFIA platform for quantifying neutralizing antibodies produced from COVID-19 vaccinations was developed, showing sensitivity and accuracy in large-scale evaluation of vaccine effectiveness.
BIOSENSORS & BIOELECTRONICS
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Article
Biotechnology & Applied Microbiology
Reda Salem et al.
Summary: This study developed a qualitative immunochromatographic test for detecting immunoglobulins M and G against SARS-CoV-2. The test showed good specificity and moderate sensitivity, making it valuable for surveillance programs in low-resource countries and monitoring antibody levels in vaccinated individuals.
BIOTECHNOLOGY LETTERS
(2022)
Article
Virology
Hongbo Liu et al.
Summary: A CRISPR/Cas-based detection method was developed to rapidly identify individuals infected with SARS-CoV-2 and control the spread of COVID-19. The method utilizes multienzyme isothermal amplification, CRISPR-Cas13a nuclease, and LFA, achieving high sensitivity and reliability.
JOURNAL OF MEDICAL VIROLOGY
(2022)
Article
Chemistry, Analytical
Gaoxing Su et al.
Summary: The orthogonal CRISPR-Cas-mediated multiplexed lateral flow assay (OC-MLFA) proposed in this study for SARS-CoV-2 genome detection takes advantage of activation and cleavage preferences between Cas12a and Cas13a, achieving high accuracy and efficiency. This method features simultaneous detection of multiple genes in one reaction and colorimetric readout through a single strip, showing promising feasibility for field-deployable point-of-care testing applications.
SENSORS AND ACTUATORS B-CHEMICAL
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Article
Chemistry, Analytical
Gaihua Cao et al.
Summary: This study developed a biosensor based on CRISPR/Cas13a and RPA technology to detect the S and Orf1ab genes of SARS-CoV-2 quickly and accurately. Additionally, an automated, portable fluorescence analyzer and lateral flow strip were developed to provide more accurate and high-throughput detection results during on-site testing.
Review
Biology
Galyah Alhamid et al.
Summary: Since its declaration as a pandemic in March 2020, COVID-19 has been the center of attention due to its rapid and uncontrollable spread. Diagnostic tests, such as RT-qPCR, are widely used for detecting SARS-CoV-2, but alternative tests are being explored to meet the global demand. This review summarizes the recent updates on SARS-CoV-2 detection methods and their limitations, aiming to guide researchers and clinicians in identifying more rapid, reliable, and sensitive diagnostic methods.
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Jian Ju et al.
Summary: A novel fluorescent lateral flow immunoassay (LFA) platform was developed for the rapid and accurate detection of SARS-CoV-2-specific antibodies. The platform employs aggregation-induced emission carbon dots conjugated with the SARS-CoV-2 spike protein, providing high sensitivity and potential applications in public health and clinical research.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
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Review
Chemistry, Analytical
Nayeli Shantal Castrejon-Jimenez et al.
Summary: This review summarizes the advantages, limitations, and evolution of lateral flow immunoassays (LFIA) during the SARS-CoV-2 pandemic and the challenges of improving these diagnostic devices.
Review
Medicine, Research & Experimental
Milad Zandi et al.
Summary: The accessory proteins of SARS-CoV-2 play significant roles in immune evasion and viral pathogenesis, making them potential targets for drug development. The IFN-I and IFN-III responses are essential for clearing the infection. Several SARS-CoV-2 accessory proteins, such as ORF3a, ORF7a, and ORF7b, inhibit the host's IFN-beta response.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Chemistry, Analytical
Bongkot Ngamsom et al.
Summary: In response to the disparities of vaccination coverage in low-and middle-income countries, this study presents a lab-on-a-chip device called 'IFAST-LAMP-CRISPR', which provides an affordable, rapid, and high-precision molecular diagnostic means for detecting SARS-CoV-2. The device integrates RNA extraction, amplification, molecular detection, and lateral flow readout in one device, offering a simple, cost-effective, and integrated platform for COVID-19 diagnostics in low-resource settings.
Article
Chemistry, Analytical
Chujun Zheng et al.
Summary: In this study, a novel magnetic lateral flow assay was developed for dual-mode detection of gastrin-17 (G-17), an important biomarker for early gastric cancer diagnosis. The assay utilized iron oxide decorated with platinum probes, which exhibited magnetic properties and peroxidase activity. The assay showed high sensitivity and specificity, and results were consistent with the enzyme-linked immunosorbent assay method. The entire testing process was simple, rapid, and suitable for portable diagnostic applications.
Article
Chemistry, Multidisciplinary
Guo-Qiang Zhang et al.
Summary: This study developed test strips for the detection of SARS-CoV-2 using AIE luminogens as reporters, achieving high sensitivity and specificity with limits of detection as low as 6.9 ng/mL for RBD protein and 7.2 ng/mL for N protein. The integration of the test strips onto N95 masks simplified the sampling procedures and showed high anti-interference capacity in complex biosamples.
CELL REPORTS PHYSICAL SCIENCE
(2022)
Article
Biochemical Research Methods
Yunxiang Wang et al.
Summary: A low-cost and highly sensitive diagnostic platform is reported, which can simultaneously distinguish wild-type (WT) SARS-CoV-2 and its two mutations within 2 hours. The platform utilizes PCR amplification and a dual-color fluorescent test strip to detect the specific nucleic acid fragments, with results interpreted by the naked eye or a smartphone-connected device. This method provides a simple, accurate, and affordable approach for screening SARS-CoV-2 and its variants, even in resource-limited settings.
Review
Immunology
Leila Mousavizadeh et al.
Summary: COVID-19 is a novel coronavirus belonging to the Betacoronavirus genus, with similarities and differences in genomic structure compared to other human Betacoronaviruses. It contains at least six open reading frames and important structural proteins are encoded near the one-third of the genome.
JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION
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Biophysics
Yaofeng Zhou et al.
Summary: The PEI-CISG technology introduced in this study controls the growth of copper nanoshells on the surface of gold nanoparticles in AuNP-LFS, effectively enhancing the detection sensitivity for various infectious diseases.
BIOSENSORS & BIOELECTRONICS
(2021)
Review
Chemistry, Multidisciplinary
Honghong Rao et al.
Summary: This article explores the importance of aggregation-induced nanozymes in enhancing the activity of nanozymes and improving the colorimetric sensing performance, as well as the future prospects and challenges in this field.
CHINESE CHEMICAL LETTERS
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Article
Chemistry, Analytical
Vasily G. Panferov et al.
Summary: Three techniques were compared to lower the LOD of SARS-CoV-2 LFIA, with gold enhancement being the most optimal, increasing the maximum dilution of samples by 500 times and providing highly sensitive and rapid testing.
Article
Biophysics
Aldo Roda et al.
Summary: The study developed a noninvasive and highly sensitive immunosensor for IgA in saliva and serum, which can be used for diagnosing and monitoring COVID-19 infection. The immunosensor demonstrated the capability to detect salivary IgA in infected individuals and correlated with disease severity and infection time.
BIOSENSORS & BIOELECTRONICS
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Article
Biophysics
Jong-Hwan Lee et al.
Summary: This study presents a novel rapid detection method for the SARS-CoV-2 S1 antigen using a matched pair of ACE2 and antibody. The detection method showed high specificity and sensitivity without cross-reactivity, making it a valuable tool in detecting the S1 antigen from COVID-19 patients.
BIOSENSORS & BIOELECTRONICS
(2021)
Review
Chemistry, Multidisciplinary
Zhukang Guo et al.
Summary: Aptamers are oligonucleotide chains with specific binding ability to protein and other targets, and can be used to discover novel tumor markers. Further characterization of newly acquired membrane proteins is essential for their clinical use as tumor markers.
CHINESE CHEMICAL LETTERS
(2021)
Article
Medical Laboratory Technology
Giulia Brisotto et al.
Summary: The study showed that SARS-CoV-2 mRNA vaccines can induce a lasting IgG response for at least 4 months, even beyond the parameters of clinical trials. The observed antibody decay at follow-up suggests further research is needed to characterize the immune response and identify individuals with low anti-SARS-CoV-2 immunity who may require a vaccination booster.
CLINICA CHIMICA ACTA
(2021)
Article
Immunology
Mohammed A. Rohaim et al.
Summary: Coronaviruses, due to their zoonotic nature, have caused various diseases in both humans and animals. The recent emergence of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causing COVID-19 has resulted in high morbidity and mortality. Understanding the structure and function of coronaviruses' RNA synthesizing machinery and replication strategies is crucial for developing effective control strategies.
MICROBIAL PATHOGENESIS
(2021)
Article
Biochemistry & Molecular Biology
Ruei-Min Lu et al.
Summary: Mitigation strategies for the COVID-19 pandemic have been hindered by the emergence of SARS-CoV-2 variants. A panel of 41 monoclonal antibodies against SARS-CoV-2 NP was generated, with 9 high-binding antibodies used in a new rapid diagnostic test showing high sensitivity and specificity for detecting viral variants. The developed LFIA strips have the capability to detect 10 NP mutants, including the alpha, beta, gamma, and delta variants.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Medical Laboratory Technology
Lin Shen et al.
Summary: In third-world countries, the lateral flow immunoassay strip (LFIA) may be a better method for detecting SARS-CoV-2, and this study developed a highly specific LFIA with a sensitivity and specificity of 98.22% and 97.93%, respectively.
JOURNAL OF CLINICAL LABORATORY ANALYSIS
(2021)
Article
Biophysics
Hye-Yeon Kim et al.
Summary: This study presents the development of a specific LFIA-based biosensor for COVID-19 using phage display technology to generate antibodies targeting SARS-CoV-2 NP antigen, allowing rapid and specific detection of the virus. The newly developed rapid diagnostic test shows potential for use in the diagnosis of COVID-19.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Biophysics
Dan Liu et al.
Summary: The nanozyme-based chemiluminescence paper assay offers a new method for rapid and sensitive detection of SARS-CoV-2 antigen. The test is specific to the SARS-CoV-2 antigen and can be completed within 16 minutes.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Biochemistry & Molecular Biology
Dan Liu et al.
Summary: The outbreak of COVID-19 has posed a significant challenge to healthcare systems worldwide, necessitating the urgent development of low-cost and convenient testing technologies.
MOLECULAR IMMUNOLOGY
(2021)
Article
Environmental Sciences
Manish Srivastava et al.
Summary: This study provides an overview of the COVID-19 pandemic and nanomaterials-enabled biosensing approaches for the diagnosis of SARS-CoV-2. Despite limited research on nanomaterials-enabled biosensing techniques, the review summarizes nanomaterials-mediated improved biosensing strategies and possible mechanisms for diagnosing COVID-19.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Chemistry, Analytical
Shiliang Chen et al.
Summary: The newly developed SERS-based LFIA strip using GERTs achieved highly sensitive detection of anti-SARS-CoV-2 IgM and IgG, with detection limits 100 times lower compared to conventional LFIA strips, improving the accuracy and reliability of early diagnosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Haifeng Liu et al.
Summary: This study reported a surface-enhanced Raman scattering-based lateral flow immunoassay method for the high sensitivity simultaneous detection of anti-SARS-CoV-2 IgM/IgG. The method utilized custom SERS tags and dual layers of Raman dye, with signal intensities easily recorded by a portable Raman instrument for high sensitivity analysis. Validation on samples from COVID-19 patients and healthy individuals showed high accuracy and specificity.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Multidisciplinary
Jiang Xu et al.
Summary: The novel microfluidic test kit combines ultra-high throughput hydrodynamic filtration and sandwich immunoassay techniques for rapid separation and enrichment of antigens, demonstrating high detection sensitivity, low cost, and suitability for areas with inadequate medical infrastructure and limited laboratory resources.
Review
Chemistry, Analytical
Yaofeng Zhou et al.
Summary: The global COVID-19 pandemic caused by the novel coronavirus has posed a serious threat to human life and health since its discovery in December 2019. Lateral flow assay (LFA) has been widely accepted as a critical point-of-care diagnostic platform for the rapid diagnosis of COVID-19, although it faces challenges and requires continuous improvement to enhance its efficiency in clinical diagnostics.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Rui Chen et al.
Summary: A new serological diagnostic method was designed for early detection of COVID-19 using AIE nanoparticles. The method showed comparable or higher sensitivity to ELISA and outperformed a commercial colloidal gold nanoparticle test strip. Early detection of IgM and IgG was achieved within 1-7 days of symptom onset with the AIE nanoparticle-based test strip.
Article
Pathology
Hin Fung Tsang et al.
Summary: The study compared the performance of cepheid (R) Xpress SARS-CoV-2 assay and cobas (R) Liat (R) SARS-CoV-2 & Influenza A/B assay in detecting SARS-CoV-2, demonstrating excellent concordance between the two systems. The study is limited by the small number of SARS-CoV-2 positive and weak-positive specimens.
EXPERT REVIEW OF MOLECULAR DIAGNOSTICS
(2021)
Review
Immunology
Akanksha Roberts et al.
Summary: A vaccine is crucial, but rapid and precise diagnostic methods are also essential in monitoring and combating the spread of SARS-CoV-2 and its variants. A variety of conventional and modern diagnostic methods have been developed, with ongoing research focusing on developing more economical tools to tackle the COVID-19 pandemic.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Anas Shamsi et al.
Summary: The emergence of SARS-CoV-2 has posed a global threat due to the continuous rise in COVID-19 cases. The complex mechanism of the virus pathogenesis hinders the development of effective therapeutics, but in-depth analysis of the genome architecture and pathogenesis may lead to potential treatment options.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Review
Biotechnology & Applied Microbiology
Yuan Jia et al.
Summary: Paper-based point-of-care tests have gained interest for their rapid, low-cost, and instrument-free detection capabilities. This review focuses on the development of paper-based POC devices for detecting SARS-CoV-2, discussing principles, performance, advantages, drawbacks, limitations, and prospects of existing devices in the field.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Medicine, General & Internal
Wen-Yeh Hsieh et al.
Summary: The COVID-19 pandemic, caused by SARS-CoV-2, poses a significant challenge to the global healthcare system. Early detection and treatment of infected cases are crucial, with rapid and cost-effective immunoassays like LFIA being utilized for quick and large-scale testing.
Article
Nanoscience & Nanotechnology
Yilin Liu et al.
Summary: The study developed an advanced LFA technology based on gold nanospheres and thermal contrast amplification for highly sensitive detection of SARS-CoV-2 virus, achieving visual detection sensitivity comparable to PCR for the first time. The use of a fast TCA reading algorithm further improved the accuracy of detection.
ACS APPLIED NANO MATERIALS
(2021)
Article
Chemistry, Analytical
Qiaoge Bayin et al.
Summary: The study introduces a novel lateral flow immunoassay method based on superparamagnetic nanoparticles and giant magnetoresistance sensing system for simultaneous quantitative detection of anti-SARS-CoV-2 immunoglobulin M and G. This method offers advantages in cost-effectiveness, time efficiency, and ease of operation.
Review
Infectious Diseases
Mumtarin Jannat Oishee et al.
Summary: This review elaborates on various diagnostic tools available or under investigation for COVID-19, including cell culture, immunofluorescent antibody, radiologic imaging, RT-PCR assay, CRISPR-Cas, microfluidics, and immunoassays like ELISA and LFIA. Advancements in NGS and metagenomics are further improving viral detection in this global challenge.
INFECTION AND DRUG RESISTANCE
(2021)
Review
Chemistry, Analytical
Riccarda Antiochia
Summary: This review summarizes the current state of paper-based biosensors (PBBs) used in the detection of COVID-19, specifically focusing on three categories for SARS-CoV-2 diagnostics. It highlights the characteristics, analytical performance, advantages and drawbacks of each type of biosensor compared to traditional methods, with the hope of assisting scientists in developing novel PBB platforms with enhanced performance to help contain the COVID-19 outbreak.
Article
Nanoscience & Nanotechnology
Chongwen Wang et al.
Summary: A dual-mode lateral flow immunoassay biosensor was developed for high-sensitivity simultaneous detection of SARS-CoV-2 spike and nucleocapsid protein antigens, offering enhanced accuracy and efficiency in viral infection screening. The system demonstrated superior performance in detecting SARS-CoV-2 antigens in saliva and nasal swab samples, with detection limits of 1 and 0.5 pg/mL for the spike and nucleocapsid protein antigens, respectively.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Chemistry, Analytical
Supriya Srivastav et al.
Summary: The study compared the performance of a conventional lateral flow assay (LFA) with a surface-enhanced Raman scattering (SERS)-based LFA test in detecting SARS-CoV-2-specific IgM/IgG in sera of COVID-19 patients. The custom-built SERS reader showed at least an order of magnitude higher sensitivity than conventional LFAs with naked-eye detection. SERS detection in a purified system showed a sensitivity that is 7 orders of magnitude higher than naked-eye detection, indicating the high potential of SERS-based LFAs in point-of-care testing.
ANALYTICAL CHEMISTRY
(2021)
Review
Chemistry, Multidisciplinary
Yuyue Xu et al.
Summary: Point-of-care nucleic acid testing (POCNAT) plays a crucial role in infectious disease outbreaks, offering rapid, simple, and automated detection of nucleic acid. Leveraging advancements in technology, novel experimental carriers like chips, cartridges, and papers have been employed for automated nucleic acid detection, marking significant progress in the field.
CHINESE CHEMICAL LETTERS
(2021)
Article
Immunology
Xu Chen et al.
Summary: The novel molecular diagnosis technique, multiplex reverse transcription loop-mediated isothermal amplification (mRT-LAMP) combined with a nanoparticle-based lateral flow biosensor, demonstrated high sensitivity and specificity in detecting the SARS-CoV-2 virus. The mRT-LAMP-LFB method is a simple, rapid, and reliable assay for identifying SARS-CoV-2, especially in resource-constrained regions for prevention and control of COVID-19.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Review
Engineering, Biomedical
Michael M. Kaminski et al.
Summary: This Review summarizes the rapidly expanding toolbox of CRISPR-based diagnostics, highlighting their applications in real-time diagnosis. It mentions that nucleic acid-based diagnostics are sensitive and specific, but most require expensive equipment and trained personnel.
NATURE BIOMEDICAL ENGINEERING
(2021)
Article
Chemistry, Analytical
Daniel W. Bradbury et al.
Summary: The innovative 3D printed casing incorporates lateral-flow immunoassay, dehydrated signal enhancement reagents, and a sealed buffer chamber, allowing for easy detection of the SARS-CoV-2 N-protein in undiluted serum in just 40 minutes.
Article
Chemistry, Analytical
Lai-Di Xu et al.
Summary: This method utilizes red emission-enhanced CD-based silica spheres to ultrasonically detect SARS-CoV-2 NP, demonstrating fast detection speed, high specificity, and ease of operation.
Article
Chemistry, Analytical
Chongwen Wang et al.
Summary: The study introduced a lateral flow immunoassay method using quantum dot nanotags for simultaneous detection of SARS-CoV-2-specific IgM and IgG in patient serum, demonstrating high sensitivity and specificity. The method showed great potential for rapid and accurate identification of infected individuals, as validated by clinical samples.
Review
Chemistry, Analytical
Chujun Zheng et al.
Summary: This review discusses two main research directions of lateral flow nucleic acid tests, one involving the incorporation of isothermal amplification methods for increased sensitivity, and the other focusing on the development of novel labeling materials for improved quantifiability. Future research will focus on integrating the entire testing process into microfluidic systems and combining with molecular diagnostic tools to facilitate rapid and accurate testing.
Article
Agriculture, Multidisciplinary
Jinmei Fu et al.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2020)
Review
Chemistry, Multidisciplinary
Buddhisha Udugama et al.
Editorial Material
Pathology
Alireza Tahamtan et al.
EXPERT REVIEW OF MOLECULAR DIAGNOSTICS
(2020)
Article
Pharmacology & Pharmacy
Wanbo Tai et al.
ANTIVIRAL RESEARCH
(2020)
Article
Medicine, General & Internal
Christian Beetz et al.
Article
Chemistry, Analytical
Sangheon Yu et al.
ANALYTICAL CHEMISTRY
(2020)
Article
Chemistry, Multidisciplinary
Alexander N. Baker et al.
ACS CENTRAL SCIENCE
(2020)
Review
Microbiology
Boris G. Andryukov
Article
Chemistry, Multidisciplinary
Lu Zeng et al.
MATERIALS CHEMISTRY FRONTIERS
(2020)
Review
Chemistry, Analytical
Zhengquan Yan et al.
Article
Chemistry, Multidisciplinary
Xiaolin Huang et al.
Article
Nanoscience & Nanotechnology
Wenqiang Yan et al.
NANO-MICRO LETTERS
(2019)
Review
Chemistry, Analytical
Qi Qin et al.
Review
Materials Science, Biomaterials
Xiaoqun Gong et al.
JOURNAL OF MATERIALS CHEMISTRY B
(2017)
Article
Chemistry, Physical
M. A. van Dijk et al.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2006)
