Development of cobalt-incorporated chitosan scaffold for regenerative potential in human dental pulp stem cells: An in vitro study

The aim of the study was to comparatively evaluate chitosan and Cobalt incorporated chitosan (CoCH) scaffold at varying concentrations in terms of their material characteristics, cytotoxicity and cell adhesion potential. In the present study, cobalt incorporated chitosan scaffolds at varying concentrations were prepared and dried. The synthesised scaffolds were characterised using XRD, FTIR, SEM-EDX and BET which revealed amorphous, porous surface of CoCH scaffolds and FTIR analysis showed the complexation confirming the chelation of cobalt with chitosan. The experimental scaffolds proved to be non-cytotoxic when compared to chitosan scaffolds on XTT analysis. Cell-seeding assay revealed enhanced adherence of hDPSCs to CoCH scaffold at 1:1 ratio in the concentration of 100 mL of 100 mu mol/L cobalt chloride solution in 100mL of 2% chitosan solution, when compared to other groups. The results highlighted that 100 mu mol/L concentration of cobalt chloride when incorporated in 1:1 ratio into 2 % CH solution yields a promising porous, biocompatible scaffold with enhanced cellular adhesion for dentin-pulp regeneration.

Automated summary

The aim of this study was to compare chitosan and cobalt incorporated chitosan scaffold in terms of material characteristics, cytotoxicity, and cell adhesion potential. The results showed that cobalt incorporated chitosan scaffold had an amorphous, porous surface and formed chelation with chitosan. XTT analysis demonstrated that the experimental scaffold was non-cytotoxic. Cell seeding assay revealed enhanced cell adhesion to the cobalt incorporated chitosan scaffold at a 1:1 ratio of 100 mL of 100 mu mol/L cobalt chloride solution in 100 mL of 2% chitosan solution.