Bach1 inhibitor HPP-D mediates ?-globin gene activation in sickle erythroid progenitors

Sickle cell disease (SCD) is the most common ss-hemoglobinopathy caused by various mutations in the adult ss-globin gene resulting in sickle hemoglobin production, chronic hemolytic anemia, pain, and progressive organ damage. The best therapeutic strategies to manage the clinical symptoms of SCD is the induction of fetal hemoglobin (HbF) using chemical agents. At present, among the Food and Drug Administration-approved drugs to treat SCD, hydroxyurea is the only one proven to induce HbF protein synthesis, however, it is not effective in all people. Therefore, we evaluated the ability of the novel Bach1 inhibitor, HPP-D to induce HbF in KU812 cells and primary sickle erythroid progenitors. HPP-D increased HbF and decreased Bach1 protein levels in both cell types. Furthermore, chromatin immunoprecipitation assay showed reduced Bach1 and increased NRF2 binding to the gamma-globin promoter antioxidant response elements. We also observed increased levels of the active histone marks H3K4Me1 and H3K4Me3 supporting an open chromatin configuration. In primary sickle erythroid progenitors, HPP-D increased gamma-globin transcription and HbF positive cells and reduced sickled erythroid progenitors under hypoxia conditions. Collectively, our data demonstrate that HPP-D induces gamma-globin gene transcription through Bach1 inhibition and enhanced NRF2 binding in the gamma-globin promoter antioxidant response elements.

Automated summary

This study evaluated the ability of a novel Bach1 inhibitor, HPP-D, to induce HbF in KU812 cells and primary sickle erythroid progenitors. The results showed that HPP-D increased HbF levels and decreased Bach1 protein levels in both cell types. Furthermore, it enhanced NRF2 binding to the gamma-globin promoter antioxidant response elements, leading to an open chromatin configuration and increased gamma-globin transcription.