Enhancing electrochemical nitrite sensing with a novel nanocomposite of activated carbon/carbon cloth derived from microbial biofilm

A novel approach was employed to fabricate a biofilm-derived activated carbon (BioAc) electrode on a carbon cloth (Cc) substrate for electrochemical nitrite sensing in water samples. The biofilm/Cc electrode was developed using a bioelectrochemical reactor, featuring a three-electrode system with nutrient media and microbial sources. The resultant biofilm electrode was activated at 450 degrees C for 2 h to eliminate impurities and enhance porosity. Morphological analysis of the BioAc/Cc electrode revealed a surface characterized by a compact film composed of numerous carbon nanoparticles. X-ray diffraction (XRD) analysis exhibited broad, highly crystalline peaks, enhancing both the electrode surface area and conductivity. Amperometry tests on the modified BioAc/Cc electrodes demonstrated a detection limit of 0.015 mu M, a sensitivity of 1946.54 mu A mM-1 cm-2, and a linear range spanning 0.35-478.21 mu M at neutral pH conditions. Moreover, the electrodes demonstrated good stability with a RSD of 2.25% after 60 days and high reproducibility with an RSD of 1.64%. Real-time results showed 99.2 and 100.1% recovery for tap water and drinking water, respectively, highlighting the potential for commercialization in the future. These findings suggest that the BioAc/Cc electrode holds substantial potential for precise nitrite detection in environmental and wastewater applications.

Automated summary

A novel biofilm-derived activated carbon electrode was successfully fabricated for electrochemical nitrite sensing in water samples. The electrode exhibited a low detection limit, high sensitivity, good stability, reproducibility, and demonstrated promising results in real-time testing, indicating its potential in environmental and wastewater applications.