Journal
FRONTIERS IN PLANT SCIENCE
Volume 7, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fpls.2016.00437
Keywords
Cucumis sativus L.; SLAF-seq; high-density genetic map; SNPs; QTL analysis
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Funding
- National Program on Key Basic Research Project of China [2012CB113900]
- Shanghai Municipal Committee of Science and Technology [13JC1403600]
- China Innovative Research Team, Ministry of Education, Shanghai Graduate Education and Innovation Program (Horticulture)
- Shanghai Municipal Agricultural Commission Project [Hu Nong Ke ZhongZi-2015-6]
- Agri-X Project of Shanghai Jiao Tong University [Agri-X2015002]
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High-density genetic linkage map plays an important role in genome assembly and quantitative trait loci (QTL) fine mapping. Since the coming of next-generation sequencing, makes the structure of high-density linkage maps much more convenient and practical, which simplifies SNP discovery and high-throughput genotyping. In this research, a high-density linkage map of cucumber was structured using specific length amplified fragment sequencing, using 153 F-2 populations of S1000 x S1002. The high-density genetic map composed 3,057 SLAFs, including 4,475 SNP markers on seven chromosomes, and spanned 1061.19 GM. The average genetic distance is 0.35 cM. Based on this high-density genome map, QTL analysis was performed on two cucumber fruit traits, fruit length and fruit diameter. There are 15 QTLs for the two fruit traits were detected.
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