Journal
FRONTIERS IN PLANT SCIENCE
Volume 7, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fpls.2016.01650
Keywords
GUN5; CHLH; Mg-chelatase; tetrapyrrole; plastid signal; stomatal ABA response
Categories
Funding
- JSPS KAKENHI [JP 24570046, JP 21570039]
- Japan Society for the Promotion of Science [22120002, 15H04389]
- Grants-in-Aid for Scientific Research [15H04389] Funding Source: KAKEN
Ask authors/readers for more resources
Expression of Photosynthesis-Associated Nuclear Genes (PhANGs) is controlled by environmental stimuli and plastid-derived signals (plastid signals) transmitting the developmental and functional status of plastids to the nucleus. Arabidopsis genomes uncoupled (gun) mutants exhibit defects in plastid signaling, leading to ectopic expression of PhANGs in the absence of chloroplast development. GUN5 encodes the plastid-localized Mg-chelatase enzyme subunit (CHLH), and recent studies suggest that CHLH is a multifunctional protein involved in tetrapyrrole biosynthesis, plastid signaling and ABA responses in guard cells. To understand the basis of CHLH multifunctionality, we investigated 15 gun5 missense mutant alleles and transgenic lines expressing a series of truncated CHLH proteins in a severe gun5 allele (cch) background (tCHLHs, 10 different versions). Here, we show that Mg-chelatase function and plastid signaling are generally correlated; in contrast, based on the analysis of the gun5 missense mutant alleles, ABA-regulated stomatal control is distinct from these two other functions. We found that none of the tCHLHs could restore plastid-signaling or Mg-chelatase functions. Additionally, we found that both the C-terminal half and N-terminal half of CHLH function in ABA-induced stomatal movement.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available