Journal
FRONTIERS IN MICROBIOLOGY
Volume 7, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fmicb.2016.01049
Keywords
syngas; methanogenesis; CODH; reductive acetyl-CoA pathway; Methanothermobacter thermoautotrophicus
Categories
Funding
- ERC [323009]
- Netherlands Ministry of Education, Culture and Science [024.002.002]
- Netherlands Science Foundation (NWO)
- European Research Council (ERC) [323009] Funding Source: European Research Council (ERC)
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Hydrogenotrophic methanogenic archaea are efficient H-2 utilizers, but only a few are known to be able to utilize CO. Methanothermobacter thermoautotrophicus is one of the hydrogenotrophic methanogens able to grow on CO, albeit about 100 times slower than on H-2 + CO2. In this study, we show that the hydrogenotrophic methanogen Methanothermobacter marburgensis, is able to perform methanogenic growth on H-2/CO2/CO and on CO as a sole substrate. To gain further insight in its carboxydotrophic metabolism, the proteome of M. marburgensis, grown on H-2/CO2 and H-2/CO2/CO, was analyzed. Cultures grown with H-2/CO2/CO showed relative higher abundance of enzymes involved in the reductive acetyl-CoA pathway and proteins involved in redox metabolism. The data suggest that the strong reducing capacity of CO negatively affects hydrogenotrophic methanogenesis, making growth on CO as a sole substrate difficult for this type of methanogens. M. marburgensis appears to partly deal with this by up-regulating co-factor regenerating reactions and activating additional pathways allowing for formation of other products, like acetate.
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