Journal
TALANTA
Volume 267, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.talanta.2023.125104
Keywords
Fluorescent probe; Biothiols; Sulfur dioxide; Bioimaging; Metabolism; Mitochondria
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A novel multi-signal near-infrared fluorescent probe was developed to distinguish biothiols and visualize the metabolism of its derivatives. It showed high sensitivity, selectivity, and rapid fluorescence response, making it a convenient and efficient tool for understanding the physiological functions of biothiols and its metabolism.
Biothiols and its metabolite SO2 derivatives play vital roles in various physiological processes. Although a few probes have been designed for monitoring the metabolism of biothiols, developing multi-signal fluorescent probes with practicability for simultaneously distinguishing biothiols (GSH, Cys and Hcy) and real-time visualizing SO2 derivatives is an enormous challenge. To better visualize biothiols metabolism in vitro and vivo, we developed a novel multi-signal NIR fluorescent probe (probe 2) with mitochondria-targeted for distinguishing biothiols and its metabolism, based on an ICT-PET synergetic mechanism. Probe 2 with dual recognition sites distinguishing detected Cys/Hcy (Red-Green), GSH (Green) and SO32 � (Blue) via three channels. First probe 2 distinguished Cys and GSH to estimate main biothiols in living cells through the ratio changes of two welldefined emission bands (Red-Green), and then imaged its metabolite SO2 with ratiometric fluorescence (RedBlue), eliminating the interference by different biothiols. Notably, probe 2 exhibits satisfactory sensitivity (detection limit: 0.21, 0.13, 0.14 and 3.06 mu M for Cys, Hcy, GSH and SO32 , respectively), high selectivity, reliability at physiological pH, and rapid fluorescence response (within 10 min). Given these advantages, probe 2 has been successfully applied to the real-time monitor GSH metabolic process in MCF-7 cells and biothiols metabolism in breast cancer, suggesting biothiols metabolic changes might be a diagnostic indicator during cancer treatment. So probe 2 is a convenient and efficient tool for understanding the physiological functions of biothiols and its metabolism.
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