Functional MRI of murine olfactory bulbs at 15.2T reveals characteristic activation patters when stimulated by different odors

Thanks to its increased sensitivity, single-shot ultrahigh field functional MRI (UHF fMRI) could lead to valuable insight about subtle brain functions such as olfaction. However, UHF fMRI experiments targeting small organs next to air voids, such as the olfactory bulb, are severely affected by field inhomogeneity problems. Spatiotemporal Encoding (SPEN) is an emerging single-shot MRI technique that could provide a route for bypassing these complications. This is here explored with single-shot fMRI studies on the olfactory bulbs of male and female mice performed at 15.2T. SPEN images collected on these organs at a 108 & mu;m in-plane resolution yielded remarkably large and well-defined responses to olfactory cues. Under suitable T2* weightings these activation-driven changes exceeded 5% of the overall signal intensity, becoming clearly visible in the images without statistical treatment. The nature of the SPEN signal intensity changes in such experiments was unambiguously linked to olfaction, via single-nostril experiments. These experiments highlighted specific activation regions in the external plexiform region and in glomeruli in the lateral part of the bulb, when stimulated by aversive or appetitive odors, respectively. These strong signal activations were non-linear with concentration, and shed light on how chemosensory signals reaching the olfactory epithelium react in response to different cues. Second-level analyses highlighted clear differences among the appetitive, aversive and neutral odor maps; no such differences were evident upon comparing male against female olfactory activation regions.

Automated summary

Single-shot ultrahigh field functional MRI (UHF fMRI) with increased sensitivity is valuable for studying subtle brain functions such as olfaction. However, small organs like the olfactory bulb are affected by field inhomogeneity problems. A technique called Spatiotemporal Encoding (SPEN) can bypass these problems and provide clear responses to olfactory cues. Experiments on male and female mice at 15.2T using SPEN images showed significant activations in specific regions when stimulated by aversive or appetitive odors, shedding light on the reaction of the olfactory epithelium to different cues.