Journal
COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 136, Issue -, Pages 1098-1106Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2015.10.022
Keywords
Controlled release; Growth factor; Fish collagen; Scaffold; PLGA microspheres
Funding
- National Natural Science Foundation of China [81501576, 51402294, 21207131]
- Natural Science Foundation of Fujian Province [2015J01341, 2013J01388]
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To design a scaffold controlled release system for skin tissue engineering, fish collagen/chitosan/chondroitin sulfate scaffolds were fabricated by freeze-drying and incorporated with bEGF-loaded PLGA microspheres (MPs). SEM showed that the scaffolds exhibited an interconnected porous structure, and the spherical MPs were uniformly distributed into the scaffolds. The higher swelling and degradation rate of scaffolds/MPs could lead to a higher diffusion rate of MPs from the scaffolds, causing an increase in the protein release. The release rate of proteins could be adjusted by the size of MPs and the ratio of collagen to chitosan of scaffolds. Circular dichroism spectroscopy and MU of bFGF after release indicated that the released bFGF retained its structural integrity and bioactivity during preparation. Cell proliferation and in vivo evaluation results suggested that the scaffolds/MPs had a good biocompatibility and an ability to promote fibroblast cell proliferation and skin tissue regeneration. These results demonstrated that this scaffold/MP controlled release system has the potential for skin tissue engineering. (C) 2015 Elsevier B.V. All rights reserved.
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