Journal
COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 135, Issue -, Pages 510-517Publisher
ELSEVIER
DOI: 10.1016/j.colsurfb.2015.07.075
Keywords
Block copolymers; Amphiphilic nanoparticles; Gene delivery; Protein delivery; Biodegradable; Polycaprolactone; Polyethylene glycol; Insulin; Heat-chill; Organic solvent free; Protein degradation; PEG-PCL; Polymer micelle; Micellar nanoparticle; Protein denaturation
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Degradation and denaturation of labile biomolecules during preparation of micelles by organic solvent at high temperature are some of the limitations for fabrication of advanced polymer based protein delivery systems. In this paper, effectiveness of heat-chill method for preparation of micelles containing large labile biomolecules was investigated using insulin as a model protein molecule. Micelles (average size, <120 nm) were prepared using amphiphilic diblock and triblock copolymers of poly(ethylene glycol) (PEG) and poly(epsilon-caprolactone) (PCL). Micelles were prepared by heating PEG-PCL block copolymers with distilled water at 60 degrees C followed by sudden chilling in an ice-water bath. Effects of molecular architecture on morphology, stability and protein loading capacity of micelles were investigated. Micelles prepared using high molecular weight block copolymers exhibited good colloidal stability, encapsulation efficiency and insulin release characteristics. Insulin retained its secondary structure after micelles preparation as confirmed by CD spectroscopic study. Furthermore, in vitro cytotoxicity test suggested that the prepared micellar nanoparticles possessed biocompatibility. In a nut shell, heat-chill method of micellar nanoparticles preparation is well suited for encapsulating labile proteins and other allied biomolecules which degrade in presence of toxic organic solvents and at elevated temperatures. (C) 2015 Published by Elsevier B.V.
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