Journal
FRONTIERS IN MICROBIOLOGY
Volume 7, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fmicb.2016.00310
Keywords
Pseudomonas aeruginosa; KPC-2; p10265-KPC; IncP-6
Categories
Funding
- Chongqing Application and Development Program [cstc2014yykfA110021]
- National Basic Research Program of China [2015CB554202]
- National High-Tech Research and Development Program of China [2014AA021402]
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Pseudomonas aeruginosa strain 10265 was recovered from a patient with pneumonia in a Chinese public hospital, and it displays the carbapenem resistance phenotype due to the acquisition of a non-conjugative but mobilizable IncP-6-type plasmid p10265-KPC. p10265-KPC carries a Tn5563-borne defective mer locus, and a novel triangle ISEc33-associated bla(Kpc-2) gene cluster without paired inverted repeats and paired direct repeats at both ends. Mobilization of this triangle ISEc33-associated element in p10265-KPC would be attributed to homologous recombination-based insertion of a foreign structure Tn3-ISApu1-orf7-ISApu2- ISKpn27-triangle bla(TEM-1)-bla(Kpc-2)-triangle ISKpn6-korC-orf6-klcA-triangle repB into a pre-existent intact ISEc33, making ISEc33 truncated at the 3' end. The previously reported pCOL-1 represents the first sequenced KPC-producing IncP-6 plasmid, while p10265-KPC is the second one. These two plasmids carry two distinct bla(Kpc-2) gene clusters, which are inserted into the different sites of the IncP-6 backbone and have different evolutionary histories of assembly and mobilization. This is the first report of identification of the IncP-6-type resistance plasmid in China.
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