Chemical and biological evaluation of biosurfactant fractions from Wickerhamomyces anomalus CCMA 0358

Biosurfactants (BS) are becoming a solution for today's world since they are considered a reasonable and eco-friendly option for use in products that require surfactants. This study aimed to evaluate the antibacterial activity of purified fractions containing biosurfactants produced by the yeast Wickerhamomyces anomalus CCMA 0358 using waste cooking oil (WCO) as substrate. Mixed fractions were separated and characterized by TLC, MPLC, GC-MS, LC-OMS, LC-SQMS, FTIR, H-1, C-13, DEPT 135, COSY, HSQC, and HMBC. The results confirmed the presence of palmitic acid and oleic acid fatty acids, derived from the core biosurfactant structure; however, the core could not be identified. The crude biosurfactant and its purified fractions were evaluated against pathogenic bacteria, and the purified fractions of the biosurfactant are more efficient at inhibitory and bactericidal activities than the crude biosurfactant. To the best of our knowledge, this is the first study that evaluated the antimicrobial activity of purified fractions of biosurfactants produced by the species Wickerhamomyces anomalus. Therefore, the purification of biosurfactants can emerge as an interesting alternative to increase the bioactivity of the compounds and ensure greater efficiency and biotechnological employability.

Automated summary

This study evaluated the antibacterial activity of purified fractions of biosurfactants produced by Wickerhamomyces anomalus CCMA 0358 using waste cooking oil as substrate. The results showed that the purified fractions exhibited higher inhibitory and bactericidal activities against pathogenic bacteria compared to the crude biosurfactant. This is the first study to evaluate the antimicrobial activity of purified biosurfactant fractions produced by Wickerhamomyces anomalus.