Journal
COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 135, Issue -, Pages 267-273Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2015.07.072
Keywords
Biocompatibility; Orientation of water; Self-assembled monolayer; Sum frequency generation; Zwitterionic SAM
Funding
- Japan Society for the Promotion of Science (JSPS) [22350101, 26188100]
- Ministry of Education, Culture, Sports, Science and Technology (MEXT), Tokyo, Japan [20106007]
- Grants-in-Aid for Scientific Research [15K04604] Funding Source: KAKEN
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A silane coupling agent having a zwitterionic end group was covalently bound to a semi-cylindrical fused silica prism for sum-frequency generation (SFG) analyses and to a flat glass for estimating biological affinity. It was found that total intensity of the O-H stretching region (3000-3600 cm(-1)) of the SFG spectrum derived from water in contact with a zwitterionic SAM-modified surface was smaller than that of SFG spectra derived from water in contact with a positively or negatively charged SAM-modified surface and a bare silica prism. These results indicated that water molecules in the vicinity of the zwitterionic SAM-modified surface are not strongly oriented in comparison with those of lopsidedly charged SAMs and bare silica. Moreover, the zwitterionic SAM surface suppressed non-specific adsorption of bovine serum albumin in contrast to the significant adsorption to lopsidedly charged SAMs and the bare cover glass. On the other hand, fibroblasts gradually adhered to the SAM surfaces and extended regardless of the electrical charge of the SAM, though the number of cells that adhered to the zwitterionic SAM was the smallest. The results strongly suggested that the charge neutralization of a solid material surface is very important for anti-biofouling properties. (C) 2015 Elsevier B.V. All rights reserved.
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