Biochemical and gene expression alterations due to individual exposure of atrazine, dichlorvos, and imidacloprid and their combination in zebrafish

In environmental toxicology, combined toxicity has emerged as an important concern. Atrazine (ATZ), dichlorvos (DIC), and imidacloprid (IMD) are the major pesticides, extensively used to control insect, flies, mosquitoes, and weed. Here, we investigate whether the exposure to three different types of pesticides individually and in combination for 24 h alters antioxidant enzyme responses in zebrafish (Danio rerio). Oxidative stress parameters (biochemical and mRNA expression), acetylcholinesterase (AChE) activity, and Metallothionein-II (MT-II) mRNA expression levels were measured. Present work includes toxicological assessment of individual and combined (CMD) exposure of ATZ (185.4 mu M), DIC (181 mu M), IMD (97.8 mu), and CMD (ATZ 92.7 mu M + DIC 90.5 mu M + IMD 48.9 mu M), in the liver, kidney, and brain of adult zebrafish. Lipid peroxidation (LPO), glutathione (GSH) content, AChE, superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activity along with mRNA expression of SOD, CAT, GPx, and MT-II were evaluated. Briefly, LPO, GSH content, the activity of AChE, and all antioxidant enzymes enhanced significantly in individual exposure, which was further altered in the CMD group. The mRNA expression of SOD, CAT, GPx, and MT-II in the liver and kidney showed significant down-regulation in all exposed groups. In the brain, significant upregulation in mRNA expression of SOD, CAT, GPx, and MT-II was observed in DIC and IMD groups, while ATZ and CMD showed significant downregulation except for GPx. Findings postulate that the CMD group exhibits synergistic toxic manifestation. The present study provides the baseline data on the combined toxic effects of pesticides and suggests regulating the use of pesticides.

Automated summary

In this study, the effect of individual and combined exposure to different pesticides on antioxidant enzyme responses in zebrafish was investigated. The results showed that the activity of all antioxidant enzymes was significantly enhanced in the individual exposure group, which was further amplified in the combined exposure group. mRNA expression levels of SOD, CAT, GPx, and MT-II were significantly down-regulated in the liver and kidney, while significant up-regulation was observed in the brain, except for GPx, in the DIC and IMD groups. The findings suggest a synergistic toxic manifestation in the combined exposure group.