4.5 Article

Chlorfenapyr Induces Cardiotoxicity by Downregulating Dap3 to Induce Mitochondrial Damage

Journal

Publisher

BIOLIFE SAS
DOI: 10.23812/j.biol.regul.homeost.agents.20233709.473

Keywords

chlorfenapyr; doxorubicin; myocardial damage; mitochondria damage; Dap3

Ask authors/readers for more resources

The study found that high concentrations of Chlo can impair cardiac function and cause mitochondrial damage by downregulating Dap3 expression.
Background: Chlorfenapyr (Chlo) is a pyrrole-based insecticide. It has proven effective in controlling a range of pests. This study examined the impact of Chlo on myocardial mitochondrial injury in mice and the underlying mechanisms involved.Methods: C57BL/6 mice were categorized into groups of saline, doxorubicin (DOX), and low, medium, and high doses of Chlo. The cardiac function of the mice in each group was evaluated using cardiac biomarkers. Hematoxylin and eosin (HE) staining was employed to observe changes in the myocardial tissue, and terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) was used to detect cardiomyocyte apoptosis. Proteins associated with mitochondrial damage were identified by western blotting. Furthermore, cardiomyocytes were isolated and divided into the Dimethyl sulfoxide (DMSO) group, medium and low Chlo groups, DOX group, and Chlo group. Transmission electron microscopy (TEM) was utilized to investigate the mitochondria, and the mitochondrial membrane potential was analyzed using a JC-1 kit. Lactate dehydrogenase (LDH) and reactive oxygen species (ROS) secretion were quantified to evaluate oxidative stress.Results: The results indicate that Chlo can impair cardiac function in vivo (p < 0.01). Chlo leads to a marked decrease in the mitochondrial membrane potential, resulting in mitochondrial damage (p < 0.05). In cardiomyocytes, Chlo significantly downregulates death-associated protein 3 (Dap3) expression (p < 0.01). However, overexpression of Dap3 effectively mitigated the oxidative stress, apoptosis, and mitochondrial damage induced by Chlo in cardiomyocytes (p < 0.05).Conclusions: Overall, high concentrations of Chlo trigger cardiotoxicity by downregulating Dap3, leading to mitochondrial damage.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.5
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available