3.8 Proceedings Paper

Analysis of bacterial DNA by surface enhanced Raman spectroscopy

Publisher

SPIE-INT SOC OPTICAL ENGINEERING
DOI: 10.1117/12.2671025

Keywords

Bacteria genome; Surface enhanced Raman spectroscopy; Silver nanoparticles; Detection of DNA

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A rapid SERS detection method using microwave synthesized silver nanoparticles (NPs) was reported for bacterial DNA, which allows for quick identification of bacterial species and genomic content.
The detection of bacterial deoxyribonucleic acid (DNA) can be helpful to quickly identify a bacterium. A rapid SERS detection of bacterial DNA using microwave synthesized silver (Ag) nanoparticles (NPs) was reported. The microwave synthesizer technique offers quick synthesis of Ag NPs providing control pressure and uniform heat. The NPs based SERS can detect the genomic content of Escherichia coli (E. coli) NC_000913.3 and Pseudomonas aeruginosa (P. aemginosa) NZ_CP012001 up to 50 ng/mu L and 40 ng/mu L respectively.

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