Journal
ENTOMOLOGICAL RESEARCH
Volume 46, Issue 5, Pages 324-328Publisher
WILEY
DOI: 10.1111/1748-5967.12175
Keywords
chitin; chitosan; housefly; Musca domestica; pupa shells
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Funding
- Korea Institute of Planning & Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (iPET) through (Agri-Bioindustry Technology Development Program)
- Ministry of Agriculture, Food and Rural Affairs (MAFRA) [315034031SB050]
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Chitins and chitosans are some of the most abundant natural polysaccharide materials, and are used to increase innate immune response and disease resistance in humans and animals. In this work, chitin and chitosan from housefly, Musca domestica, pupa shells were obtained by treatment with HCl and NaOH. For chitin extraction, 2N HCl and 1.25N NaOH solutions were used to achieve decalcification and deproteinization, respectively. For chitosan extraction, 50% NaOH solution was used to achieve deacetylation. The yields of chitin and chitosan from pupa shells of M. domestica were 8.02% and 5.87%, respectively. The deacetylations of chitosan (from chitinC1 and C2) were 89.76% and 92.39%, respectively, after the first alkali treatment with 50% NaOH (w/w) solution at 105 degrees C for 3 h and 5 h, respectively. The viscosities of the chitosans (from chitin C1 and C2) were 33.6 and 19.2 cP, respectively.
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