Journal
INFECTIOUS DISEASES AND CLINICAL MICROBIOLOGY
Volume 5, Issue 3, Pages 257-261Publisher
DOC DESIGN INFORMATICS CO LTD
DOI: 10.36519/idcm.2023.246
Keywords
COVID-19; SARS-CoV-2; B cell subtypes; mRNA vaccine; inactivated vaccine
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This study investigated the changes in B cell subpopulations after homologous or heterologous COVID-19 boosters. The results showed that there was a significant increase in memory B cell expression one month after BNT162b2 and CoronaVac boosters. The expression of IgG-expressing memory B cells was significantly higher with BNT162b2 than with CoronaVac booster in one month. In addition, the ratio of effector B cells was significantly higher in the first month after CoronaVac booster compared to the BNT162b2 booster.
In this study, we aimed to investigate the changes in the B cell subpopulations after homologous or heterologous COVID-19 boosters. Blood samples were collected after baseline (3-5 months after two doses of CoronaVac), 1 and 3 months after BNT162b2 (n=28 and n=6), and CoronaVac (n=7 and n=4) boosters. Peripheral blood mononuclear cells (PBMCs) were isolated and stained with B cell markers, the ratios of naive (CD19+CD20+CD27-), memory (CD19+CD20+CD27+), memory B cells expressing IgG (CD19+CD20+CD27+IgG+), and effector memory B cells (CD19+CD20+CD27+CD38+) were identified with flow cytometry. Significantly higher expression of memory B cells was observed in one month with BNT162b2 (12.16% one month, 5.98% three months) and CoronaVac (14.18% one month, 9.00% three months) boosters. IgG expressing memory B cell expression was significantly higher with BNT162b2 than with CoronaVac booster in one month (22.70% and 13.95%, respectively). The ratio of effector B cells in the first month after CoronaVac booster (25.44%) was significantly higher than the BNT162b2 booster (9.90%, p=0.0263).
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