Journal
COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 126, Issue -, Pages 426-436Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2014.12.046
Keywords
alpha-Eleostearic acid; Flow cytometry; Nanoemulsion; Reactive oxygen species; Transmission electron microscopy
Funding
- Indian Council of Medical Research grant
- Centre for Research in Nanoscience and Nanotechnology, University of Calcutta infrastructure
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The objective of the present study was to fabricate and monitor real-time, impact of a stable conjugated linolenic acid, a-eleostearic acid (ESA) rich nanoemulsion (NE) formulation (d < 200 nm) vis-a-vis ESA conventional emulsion (CE) system in ex vivo systems against both endogenous and exogenous reactive oxygen species (ROS). Accordingly, stable nanoemulsion formulation of ESA was engineered with the aid of bitter melon seed oil and non-toxic excipients. Morphology and particle size of the emulsion formulations were studied to validate stability. The real-time rapid uptake of the ESA NE and its increased prophylactic efficacy against induced endogenous and exogenous ROS in terms of cell viability and membrane integrity was evaluated flow-cytometrically and with fluorescence microscopic analysis of different primary cells. It was found that the fabricated non-toxic ESA NE had stable parameters (hydrodynamic mean diameter, particle size distribution and zeta potential) for over 12 weeks. Further, ESA NE at a concentration of similar to 70 mu M exhibited maximum efficacy in protecting cells from oxidative damage against both endogenous and exogenous ROS in lymphocytes and hepatocytes as compared to its corresponding presence in the CE formulation. This study provides a real-time empirical evidence on the influence of nano formulation in enhancing bioavailability and antioxidative properties of ESA. (C) 2015 Elsevier B.V. All rights reserved.
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