Journal
ELIFE
Volume 5, Issue -, Pages -Publisher
ELIFE SCIENCES PUBLICATIONS LTD
DOI: 10.7554/eLife.22238
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Funding
- National Institute of Diabetes and Digestive and Kidney Diseases [DK18849, DK18024]
- Howard Hughes Medical Institute
- Paul D. Wellstone Muscular Dystrophy Cooperative Research Center [1U54NS053672]
- National Natural Science Foundation of China [31570735]
- National Key Research and Development Plan of China [2016YFC0906000]
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The 'pseudokinase' SgK196 is a protein O-mannose kinase (POMK) that catalyzes an essential phosphorylation step during biosynthesis of the laminin-binding glycan on alpha-dystroglycan. However, the catalytic mechanism underlying this activity remains elusive. Here we present the crystal structure of Danio rerio POMK in complex with Mg2+ ions, ADP, aluminum fluoride, and the GalNAc-beta 3-GlcNAc-beta 4-Man trisaccharide substrate, thereby providing a snapshot of the catalytic transition state of this unusual kinase. The active site of POMK is established by residues located in non-canonical positions and is stabilized by a disulfide bridge. GalNAc-beta 3-GlcNAc-beta 4-Man is recognized by a surface groove, and the GalNAc-beta 3-GlcNAc moiety mediates the majority of interactions with POMK. Expression of various POMK mutants in POMK knockout cells further validated the functional requirements of critical residues. Our results provide important insights into the ability of POMK to function specifically as a glycan kinase, and highlight the structural diversity of the human kinome.
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