Journal
PLANT JOURNAL
Volume 116, Issue 5, Pages 1342-1354Publisher
WILEY
DOI: 10.1111/tpj.16428
Keywords
potato (Solanum tuberosum); miraculin-like proteins; Ralstonia solanacearum; vascular bundles; bacterial wilt resistance
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This study identified a gene StMLP1 in potato that belongs to the Kunitz trypsin inhibitor (KTI) family and has a role in defending against Ralstonia solanacearum (R. solanacearum) infection. The StMLP1 gene exhibits trypsin inhibitor activity and its overexpression enhances resistance to R. solanacearum, while inhibiting its expression accelerates infection. Furthermore, StMLP1 is involved in potato immunity.
Miraculin-like proteins (MLPs), members of the Kunitz trypsin inhibitor (KTI) family that are present in various plants, have been discovered to have a role in defending plants against pathogens. In this study, we identified a gene StMLP1 in potato that belongs to the KTI family. We found that the expression of StMLP1 gradually increases during Ralstonia solanacearum (R. solanacearum) infection. We characterized the promoter of StMLP1 as an inducible promoter that can be triggered by R. solanacearum and as a tissue-specific promoter with specificity for vascular bundle expression. Our findings demonstrate that StMLP1 exhibits trypsin inhibitor activity, and that its signal peptide is essential for proper localization and function. Overexpression of StMLP1 in potato can enhance the resistance to R. solanacearum. Inhibiting the expression of StMLP1 during infection accelerated the infection by R. solanacearum to a certain extent. In addition, the RNA-seq results of the overexpression-StMLP1 lines indicated that StMLP1 was involved in potato immunity. All these findings in our study reveal that StMLP1 functions as a positive regulator that is induced and specifically expressed in vascular bundles in response to R. solanacearum infection.
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