3.8 Review

Computer-aided engineering and additive manufacturing for bioreactors in tissue engineering: State of the art and perspectives

Related references

Note: Only part of the references are listed.
Article Multidisciplinary Sciences

An automated 3D-printed perfusion bioreactor combinable with pulsed electromagnetic field stimulators for bone tissue investigations

Stefano Gabetti et al.

Summary: In bone tissue engineering research, bioreactors designed with automation can improve reproducibility and overall quality of the culture process. This study presents an automated perfusion bioreactor combined with pulsed electromagnetic field (PEMF) stimulation for culturing 3D bone tissue models. Perfused cultures showed increased release of early differentiation marker alkaline phosphatase, and bidirectional perfusion favored extracellular matrix deposition. Combined with PEMF stimulation, the expression of osteogenic gene collagen type I was significantly up-regulated, indicating a positive synergic effect of the combined physical stimulations.

SCIENTIFIC REPORTS (2022)

Article Engineering, Biomedical

Personalized Volumetric Tissue Generation by Enhancing Multiscale Mass Transport through 3D Printed Scaffolds in Perfused Bioreactors

David P. Forrestal et al.

Summary: This study presents a multiscale framework combining computational fluid dynamics, additive manufacturing, and a perfusion bioreactor system for engineering personalized volumetric tissue. By designing microscale 3D printed scaffold pore geometries and customizing perfusion bioreactors, tissue growth shaped like human calcaneus bones was achieved.

ADVANCED HEALTHCARE MATERIALS (2022)

Review Engineering, Biomedical

Bioreactor design and validation for manufacturing strategies in tissue engineering

Diana Lim et al.

Summary: Regenerative medicine and tissue engineering provide new therapeutic options for restoring, maintaining, or improving tissue function. To optimize the biological function of tissue-engineered clinical products, specific conditions must be maintained in a bioreactor to allow product maturation and mimic the in vivo environment. Real-time monitoring of product functional capacity is critical for quality management during manufacturing.

BIO-DESIGN AND MANUFACTURING (2022)

Article Biotechnology & Applied Microbiology

Multiphysics simulation of a compression-perfusion combined bioreactor to predict the mechanical microenvironment during bone metastatic breast cancer loading experiments

Boyuan Liu et al.

Summary: This study found that mechanical signals in the skeleton can affect bone metastatic breast cancer cells, shedding light on how increased mechanical loading may confer anti-tumorigenic effects during metastasis.

BIOTECHNOLOGY AND BIOENGINEERING (2021)

Article Nanoscience & Nanotechnology

Critical Frequency and Critical Stretching Rate for Reorientation of Cells on a Cyclically Stretched Polymer in a Microfluidic Chip

Tianjiao Mao et al.

Summary: The study used a two-layer microfluidic chip to observe human mesenchymal stem cells under different frequencies and amplitudes of cyclic stretching, revealing that cells reorient perpendicular to the stretching direction at frequencies above a critical value, which is also amplitude-dependent. The concept of critical stretching rate was introduced to unify the frequency and amplitude dependences, with the study finding a value consistent with actin filament turnover time, suggesting a role for supramolecular relaxation in cell mechanotransduction. The theoretical calculation based on a tensegrity model matched the experimental results, providing insights into the crucial role of critical frequency and critical stretching rate in regulating cell behavior under biomechanical stimuli on biomaterials.

ACS APPLIED MATERIALS & INTERFACES (2021)

Article Chemistry, Analytical

Microfluidic Chip with Low Constant-Current Stimulation (LCCS) Platform: Human Nucleus Pulposus Degeneration In Vitro Model for Symptomatic Intervertebral Disc

An-Gi Kim et al.

Summary: Intervertebral disc degeneration causing low back pain is mediated by matrix degradation and inflammatory responses, particularly interleukin-1 beta secretion in nucleus pulposus cells. A custom electrical stimulation platform was developed to apply low constant current stimulation to microfluidic chips, showing positive modulation of inflamed human NP cells' morphological phenotype and kinetic properties. This suggests the potential for LCCS in treating degenerative diseases and its applicability for electroceuticals research.

MICROMACHINES (2021)

Article Chemistry, Multidisciplinary

Direct Perfusion Improves Redifferentiation of Human Chondrocytes in Fibrin Hydrogel with the Deposition of Cartilage Pericellular Matrix

Alexandre Dufour et al.

Summary: In this study, a combination of soluble factors, fibrin hydrogel, direct perfusion, and human articular chondrocytes was used to engineer large cartilage tissues. Perfusion preconditioning improved the quality and spatial distribution of the cartilage matrix, resulting in the reconstruction of hyaline cartilage and increased production of the main component of the pericellular matrix by chondrocytes.

APPLIED SCIENCES-BASEL (2021)

Article Engineering, Biomedical

A microphysiological system combining electrospun fibers and electrical stimulation for the maturation of highly anisotropic cardiac tissue

Adrian Lopez-Canosa et al.

Summary: The creation of cardiac tissue models for preclinical testing remains a challenge due to the limitations in replicating the complexity of cardiac tissue in vitro. In this study, a microfluidic platform was developed to provide signaling cues to immature cardiac cells, guiding them towards an adult phenotype. The platform demonstrated control over the anisotropy of cardiac tissue and induced the upregulation of key genes through electrical stimulation, validating its potential as a powerful tool for tissue engineering.

BIOFABRICATION (2021)

Article Chemistry, Multidisciplinary

3D Bioprinting of Engineered Tissue Flaps with Hierarchical Vessel Networks (VesselNet) for Direct Host-To-Implant Perfusion

Ariel A. Szklanny et al.

Summary: Creating engineered hierarchical vasculatures is crucial for implantable functional thick tissues. This study introduces a combined approach using millimetric vessel-like scaffolds and 3D bioprinted vascularized tissues to achieve fully engineered hierarchical vascular constructs for implantation. The use of sacrificial molds, endothelial cells, and vascularizing hydrogels enables the formation of functional vessels within the engineered tissue, promoting tissue perfusion and host vasculature ingrowth.

ADVANCED MATERIALS (2021)

Article Engineering, Biomedical

A novel organ-chip system emulates three-dimensional architecture of the human epithelia and the mechanical forces acting on it

Antonio Varone et al.

Summary: This study presents a new 3D cell-culture system that combines Organ-Chip technology with advantages of three-dimensional organotypic culture. Using microfabrication techniques, a flexible chip was engineered to recreate critical elements of native tissue architecture, offering researchers the possibility to model organs and their functionality more accurately. The Open-Top Chip design successfully addresses common challenges associated with Organs-on-Chip technology, demonstrating its feasibility with primary human skin and alveolar epithelial cells.

BIOMATERIALS (2021)

Article Biochemistry & Molecular Biology

Impact of Uniaxial Stretching on Both Gliding and Traction Areas of Tendon Explants in a Novel Bioreactor

Mersedeh Tohidnezhad et al.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2020)

Article Biotechnology & Applied Microbiology

Establishment and Application of Peristaltic Human Gut-Vessel Microsystem for Studying Host-Microbial Interaction

Bolin Jing et al.

FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY (2020)

Article Materials Science, Multidisciplinary

Fabrication and Functionalization of 3D Printed Polydimethylsiloxane-Based Microfluidic Devices Obtained through Digital Light Processing

Gustavo Gonzalez et al.

ADVANCED MATERIALS TECHNOLOGIES (2020)

Article Engineering, Biomedical

3D-Printed Bioreactor Enhances Potential for Tendon Tissue Engineering

Brittany L. Banik et al.

REGENERATIVE ENGINEERING AND TRANSLATIONAL MEDICINE (2020)

Article Chemistry, Analytical

Microbioreactor for lower cost and faster optimisation of protein production

Mayur Parekh et al.

ANALYST (2020)

Article Cell & Tissue Engineering

A universal multi-platform 3D printed bioreactor chamber for tendon tissue engineering

Adam J. Janvier et al.

JOURNAL OF TISSUE ENGINEERING (2020)

Article Cell Biology

Mechanical loading stimulates hypertrophy in tissue-engineered skeletal muscle: Molecular and phenotypic responses

Kathryn W. Aguilar-Agon et al.

JOURNAL OF CELLULAR PHYSIOLOGY (2019)

Article Cell & Tissue Engineering

Load-induced osteogenic differentiation of mesenchymal stromal cells is caused by mechano-regulated autocrine signaling

Sophie Schreivogel et al.

JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE (2019)

Article Biotechnology & Applied Microbiology

Numerical Investigations of Hepatic Spheroids Metabolic Reactions in a Perfusion Bioreactor

Fatemeh Sharifi et al.

FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY (2019)

Article Engineering, Mechanical

3D printed micro-mechanical device (MMD) for in situ tensile testing of micro/nanowires

Yuejiao Wang et al.

EXTREME MECHANICS LETTERS (2019)

Article Materials Science, Biomaterials

Conductive nanostructured Si biomaterials enhance osteogeneration through electrical stimulation

Yan Huang et al.

MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS (2019)

Article Chemistry, Analytical

Fabrication of integrated microfluidic devices by direct ink writing (DIW) 3D printing

Terry Ching et al.

SENSORS AND ACTUATORS B-CHEMICAL (2019)

Review Cardiac & Cardiovascular Systems

Shear stress: An essential driver of endothelial progenitor cells

Anton G. Kutikhin et al.

JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY (2018)

Article Chemistry, Physical

Instrumented cardiac microphysiological devices via multimaterial three-dimensional printing

Johan U. Lind et al.

NATURE MATERIALS (2017)

Article Biotechnology & Applied Microbiology

Industrialization of a perfusion bioreactor: Prime example of a non-straightforward process

G. Talò et al.

Journal of Tissue Engineering and Regenerative Medicine (2017)

Article Chemistry, Analytical

Simplified fabrication of integrated microfluidic devices using fused deposition modeling 3D printing

Gabriel Gaal et al.

SENSORS AND ACTUATORS B-CHEMICAL (2017)

Article Biophysics

A triphasic constrained mixture model of engineered tissue formation under in vitro dynamic mechanical conditioning

Joao S. Soares et al.

BIOMECHANICS AND MODELING IN MECHANOBIOLOGY (2016)

Article Engineering, Biomedical

3D Printed Vascular Networks Enhance Viability in High-Volume Perfusion Bioreactor

Owen Ball et al.

ANNALS OF BIOMEDICAL ENGINEERING (2016)

Article Biochemical Research Methods

Direct current electrical stimulation chamber for treating cells in vitro

Sahba Mobini et al.

BIOTECHNIQUES (2016)

Article Engineering, Biomedical

An Experimental and Computational Investigation of Bone Formation in Mechanically Loaded Trabecular Bone Explants

E. Birmingham et al.

ANNALS OF BIOMEDICAL ENGINEERING (2016)

Article Biochemical Research Methods

3D printed microfluidic devices with integrated valves

Chad I. Rogers et al.

BIOMICROFLUIDICS (2015)

Article Biochemical Research Methods

3D-printed microfluidic automation

Anthony K. Au et al.

LAB ON A CHIP (2015)

Article Multidisciplinary Sciences

Controlled electromechanical cell stimulation on-a-chip

Andrea Pavesi et al.

SCIENTIFIC REPORTS (2015)

Review Engineering, Chemical

Bioreactor Systems for Human Bone Tissue Engineering

Martina Sladkova et al.

PROCESSES (2014)

Article Chemistry, Analytical

A 3D Printed Fluidic Device that Enables Integrated Features

Kari B. Anderson et al.

ANALYTICAL CHEMISTRY (2013)

Review Chemistry, Multidisciplinary

3D Printing for Tissue Engineering

Dylan Jack Richards et al.

ISRAEL JOURNAL OF CHEMISTRY (2013)

Review Engineering, Biomedical

Whole-Organ Tissue Engineering: Decellularization and Recellularization of Three-Dimensional Matrix Scaffolds

Stephen F. Badylak et al.

ANNUAL REVIEW OF BIOMEDICAL ENGINEERING, VOL 13 (2011)

Article Biotechnology & Applied Microbiology

Supply of Nutrients to Cells in Engineered Tissues

Jeroen Rouwkema et al.

Biotechnology & Genetic Engineering Reviews (2011)

Article Biophysics

Simulation of bone tissue formation within a porous scaffold under dynamic compression

Jean-Louis Milan et al.

BIOMECHANICS AND MODELING IN MECHANOBIOLOGY (2010)

Article Cell & Tissue Engineering

Optical Mapping of Impulse Propagation in Engineered Cardiac Tissue

Milica Radisic et al.

TISSUE ENGINEERING PART A (2009)

Article Biophysics

A numerical model of heterogeneous surface strains in polymer scaffolds

Elbert Baas et al.

JOURNAL OF BIOMECHANICS (2008)

Article Biochemistry & Molecular Biology

Perfusion-decellularized matrix: using nature's platform to engineer a bioartificial heart

Harald C. Ott et al.

NATURE MEDICINE (2008)

Review Engineering, Biomedical

Bioreactors for tissue mass culture: Design, characterization, and recent advances

Y Martin et al.

BIOMATERIALS (2005)

Article Engineering, Biomedical

Electrical stimulation of denervated muscles:: First results of a clinical study

M Mödlin et al.

ARTIFICIAL ORGANS (2005)

Review Biotechnology & Applied Microbiology

Bioreactor design for tissue engineering

R Portner et al.

JOURNAL OF BIOSCIENCE AND BIOENGINEERING (2005)

Article Multidisciplinary Sciences

Functional assembly of engineered myocardium by electrical stimulation of cardiac myocytes cultured on scaffolds

M Radisic et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2004)

Article Cell & Tissue Engineering

Design of a flow perfusion bioreactor system for bone tissue-engineering applications

GN Bancroft et al.

TISSUE ENGINEERING (2003)

Review Medicine, General & Internal

Mechanobiology and diseases of mechanotransduction

DE Ingber

ANNALS OF MEDICINE (2003)