Journal
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
Volume 679, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.colsurfa.2023.132606
Keywords
PAMAM; Laccase; 4-DCP; 2
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Polyaminoamide (PAMAM) dendrimers with different generations were successfully grafted on Fe3O4-SiO2 composite particles, and laccase was immobilized to the surface using covalent cross-linking. The immobilized laccase showed excellent catalytic degradation ability for 2,4-dichlorophenol (2,4-DCP) with high stability.
Polyaminoamide (PAMAM) dendrimers with different generations from 1 G to 5 G were grafted on magnetic core-shell Fe3O4-SiO2 composite particles, respectively. Using Fe3O4 as a magnetic center and SiO2 as the connecting layer, the PAMAM dendrimers with amino functional groups were modified on SiO2 successfully, while laccase was immobilized to the surface of Fe3O4-SiO2-PAMAMx by covalent cross-linking using amide bond. Under the optimal conditions, the loading capacity of laccase reached 65.5 mg/g, and about 62.8 % of its initial activity of free laccase was reserved. Immobilized laccase degradated 2,4-dichlorophenol (2,4-DCP) with a degradation ratio of 91.5 % after 24 h. The degradation ratio of 82.9 % was maintained after 5 cycles of the operation, and when enhanced to the 10 cycles degradation, the degradation rate was still remained close to 80 %. Compared with free laccase, the comprehensive stability of immobilized laccase was improved significantly, and it displayed the excellent catalytic degradation ability for 2,4-DCP.
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