☆ 4.6 Article

Effective synthesis, development and application of a highly fluorescent cyanine dye for antibody conjugation and microscopy imaging

ORGANIC & BIOMOLECULAR CHEMISTRY (2023)

Related references

Note: Only part of the references are listed.

Article Chemistry, Analytical

Two-photon fluorescent chemosensors based on the GFP-chromophore for the detection of Zn2+ in biological samples - From design to application

Attila Csomos et al.

Summary: A library of fluorescent GFP chromophore based chemosensors (GFZnPs) was prepared for the detection of Zn2+ using two-photon microscopy. These sensors exhibit high selectivity, excellent water solubility, and wide pH-range usability, making them one of the best two-photon probes for Zn2+.

SENSORS AND ACTUATORS B-CHEMICAL (2024)

Add to Collection

Article Chemistry, Medicinal

Synthesis and biological evaluation of theranostic Trastuzumab-SN38 conjugate for Near-IR fluorescence imaging and targeted therapy of HER2+breast cancer

Dmytro Kobzev et al.

Summary: Here, researchers developed a new theranostic antibody drug conjugate (ADC) that combines a HER2-specific antibody (trastuzumab) with a near infrared dye (Cy5) and a bioactive metabolite of irinotecan (SN38). The ADC showed specific accumulation and nanomolar anti-breast cancer activity on HER2-positive cell lines. In in vivo studies, the ADC exhibited good targeting ability for HER2+ tumors and higher anticancer potency compared to trastuzumab alone or a mixture of trastuzumab with SN38. The glutathione-responsive self-immolative disulfide carbamate linker used in this study was proven to be successful and could be applied in targeted anticancer therapy.

EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY (2023)

Add to Collection

Article Chemistry, Multidisciplinary

Synthesis and Application of Two-Photon Active Fluorescent Rhodol Dyes for Antibody Conjugation and In Vitro Cell Imaging

Denes Szepesi Kovacs et al.

Summary: A novel family of julolidine-containing fluorescent rhodolsequipped with a wide variety of substituents was synthesized using a versatile two-step process. The synthesized compounds were fully characterized and demonstrated excellent fluorescence properties for microscopy imaging. The best candidate was conjugated to the therapeutic antibody trastuzumab through a copper-free strain-promoted azide-alkyne click reaction. The rhodol-labeled antibody was successfully used for in vitro confocal and two-photon microscopy imaging of Her2+ cells.

ACS OMEGA (2023)

Add to Collection

Article Biochemical Research Methods

Cyanine Masking: A Strategy to Test Functional Group Effects on Antibody Conjugate Targeting

Ek Raj Thapaliya et al.

Summary: This study systematically investigates the impact of various functional groups on the properties of antibody-fluorophore conjugates, and suggests that zwitterionic substituents possess an optimal combination of high tumor signal, tumor-to-background ratio, and low liver-to-background ratio.

BIOCONJUGATE CHEMISTRY (2022)

Add to Collection

Article Biochemical Research Methods

One-Step Conversion of NHS Esters to Reagents for Site-Directed Labeling of IgG Antibodies

Rikke A. Hansen et al.

Summary: Antibody conjugates are widely used in diagnostics, therapeutics, and molecular biology. However, the current method of using NHS esters to prepare these conjugates leads to heterogeneous products. This study presents the development of a reagent that can be activated with a chosen NHS ester and used for site-directed labeling of antibodies in a single step. The reagent demonstrates similar ease of use as NHS esters but with improved site-directed labeling.

BIOCONJUGATE CHEMISTRY (2022)

Add to Collection

Article Chemistry, Organic

Near-infrared fluorescent probes based on a quinoxaline skeleton for imaging nucleic acids in mitochondria

Hai-Yan Peng et al.

Summary: In this paper, cationic probes 1a and 1b and a neutral dye 1c were designed and synthesized for the detection of nucleic acids. The probes showed excellent selectivity and an ON-OFF fluorescence response to DNA and RNA with linear correlation between fluorescence intensity and concentration. The probes also demonstrated potential for staining nucleic acids in mitochondria, highlighting their usefulness in low toxicity mitochondrial nucleic acid probes.

ORGANIC & BIOMOLECULAR CHEMISTRY (2022)

Add to Collection

Review Chemistry, Multidisciplinary

Cyanine Dyes Containing Quinoline Moieties: History, Synthesis, Optical Properties, and Applications

Kristina Ilina et al.

Summary: This review article focuses on discussing the history, various synthetic routes, optical properties, and applications of cyanine dyes carrying quinoline moieties in medicine, pharmacology, and engineering.

CHEMISTRY-A EUROPEAN JOURNAL (2021)

Add to Collection

Article Chemistry, Organic

Synthesis and Fluorescence Mechanism of the Aminoimidazolone Analogues of the Green Fluorescent Protein: Towards Advanced Dyes with Enhanced Stokes Shift, Quantum Yield and Two-Photon Absorption

Ervin Kovacs et al.

Summary: Novel small-molecular analogues of the green fluorescence protein (GFP) chromophore were synthesised to expand and improve this fluorophore family and deepen the understanding of their fluorescence mechanism. The introduction of specific substituents and manipulation of the molecular structure successfully enhanced fluorescence emission intensity and altered spectral properties.

EUROPEAN JOURNAL OF ORGANIC CHEMISTRY (2021)

Add to Collection

Article Multidisciplinary Sciences

PharmacoSTORM nanoscale pharmacology reveals cariprazine binding on Islands of Calleja granule cells

Susanne Prokop et al.

Summary: The PharmacoSTORM single-molecule imaging technique combines fluorescent ligands and immunolabeling to provide a new approach for molecular pharmacology research at the cellular and subcellular levels, enabling precise quantification of drug-target interactions. By studying cariprazine binding to D3 dopamine receptors, the technique allows for accurate measurement of drug-target interactions and sheds light on its neurobiological substrate.

NATURE COMMUNICATIONS (2021)

Add to Collection

Article Chemistry, Physical

Molecular and Spectroscopic Characterization of Green and Red Cyanine Fluorophores from the Alexa Fluor and AF Series**

Christian Gebhardt et al.

Summary: The application of fluorescence techniques has had a significant impact on various research fields, facilitated by the development of optimized commercial dyes. However, the chemical structures of dyes and linkers used for bioconjugation often remain confidential, posing potential problems for research applications. This study investigates the molecular structures and spectroscopic properties of certain dyes, proposes structures for Alexa Fluor 555 and refines that of AF555, as well as compares the performance of different dyes in various applications.

CHEMPHYSCHEM (2021)

Add to Collection

Article Chemistry, Organic

A Comprehensive Study of the Ca2+ Ion Binding of Fluorescently Labelled BAPTA Analogues

Attila Csomos et al.

Summary: The comprehensive study on novel alterations in the structure of BAPTA revealed significant changes in the characteristics of the compounds, such as the effect of linker's length, the number of oxygen atoms in the chain, and removal of one of the rings. The results showed that even without the second aromatic ring, the fluorescence could still be turned on, and successful sensing could be achieved by replacing the chain with a single oxygen atom.

EUROPEAN JOURNAL OF ORGANIC CHEMISTRY (2021)

Add to Collection

Review Pharmacology & Pharmacy

Potential of Cyanine Derived Dyes in Photodynamic Therapy

Natalia Lange et al.

Summary: Photodynamic therapy (PDT) is an effective cancer treatment method that utilizes cyanine dyes and their derivatives as photosensitizers. Structural modifications of cyanine dyes aim to increase solubility in aqueous media, enhance phototoxicity, and improve treatment efficacy.

PHARMACEUTICS (2021)

Add to Collection

Article Chemistry, Multidisciplinary

Theoretical Design, Synthesis, and In Vitro Neurobiological Applications of a Highly Efficient Two-Photon Caged GABA Validated on an Epileptic Case

Balazs Chiovini et al.

Summary: This paper introduces a new cage-GABA compound, iDMPO-DNI-GABA, which is currently the only photoreagent for GABA uncaging without experimental compromises. Through systematic theoretical design and synthesis, the best reagent exhibits high two-photon efficiency within the 700-760 nm range, demonstrating excellent pharmacological properties suitable for complex epileptic studies.

ACS OMEGA (2021)

Add to Collection

Review Chemistry, Organic

Strategies for organelle targeting of fluorescent probes

Jiarun Lin et al.

Summary: Fluorescent tools are crucial for studying organelles' distinct chemical microenvironments. While many fluorescent tools have been developed, most only target a few organelles, leaving less-studied organelles with limited robust targeting strategies. Developing new, robust strategies is essential for expanding the effective study of organelles and creating new tools.

ORGANIC & BIOMOLECULAR CHEMISTRY (2021)

Add to Collection

Article Oncology

Evaluation of Near-infrared Fluorescence-conjugated Peptides for Visualization of Human Epidermal Receptor 2-overexpressed Gastric Cancer

Kyoungyun Jeong et al.

Summary: A HER2-specific NIR fluorescence probe was synthesized and evaluated for cancer-specific image guided surgery using an animal model. Trastuzumab-IRDye800CW exhibited high affinity for HER2 and was suitable for tumor imaging, while Affibody-IRDye800CW may require modifications to enhance tumor recognition.

JOURNAL OF GASTRIC CANCER (2021)

Add to Collection

Article Chemistry, Multidisciplinary

Synthesis and characterization of new fluorescent boro-β-carboline dyes

Denes Szepesi Kovacs et al.

Summary: The first representatives of a new fluorescent boro-beta-carboline family were synthesized by inserting difluoroboranyl group into oxaza or diaza core, exhibiting good photophysical properties with fine Stokes-shifts. Quantum chemical computations suggested improved properties of diazaborinino-carbolines over oxazaborolo-carbolines. This chemotype was nominated as a new fluorophore for developing fluorescent probes.

RSC ADVANCES (2021)

Add to Collection

Review Oncology

Antibody-Drug Conjugates: A Comprehensive Review

Puregmaa Khongorzul et al.

MOLECULAR CANCER RESEARCH (2020)

Add to Collection

Review Chemistry, Organic

Near-infrared heptamethine cyanines (Cy7): from structure, property to application

Lan Feng et al.

ORGANIC & BIOMOLECULAR CHEMISTRY (2020)

Add to Collection

Article Chemistry, Multidisciplinary

Cyclooctatetraene-conjugated cyanine mitochondrial probes minimize phototoxicity in fluorescence and nanoscopic imaging

Zhongtian Yang et al.

CHEMICAL SCIENCE (2020)

Add to Collection

Article Chemistry, Multidisciplinary

Cyanine dye mediated mitochondrial targeting enhances the anti-cancer activity of small-molecule cargoes

Alexander R. Noedling et al.

CHEMICAL COMMUNICATIONS (2020)

Add to Collection

Article Chemistry, Multidisciplinary

Cysteine specific bioconjugation with benzyl isothiocyanates

Laszlo Petri et al.

RSC ADVANCES (2020)

Add to Collection

Article Spectroscopy

Synthesis and spectroscopic characterization of novel GFP chromophore analogues based on aminoimidazolone derivatives

Attila Jancso et al.

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY (2019)

Add to Collection

Article Multidisciplinary Sciences

Amino-isocyanoacridines: Novel, Tunable Solvatochromic Fluorophores as Physiological pH Probes

Miklos Nagy et al.

SCIENTIFIC REPORTS (2019)

Add to Collection

Article Oncology

Fluorescence Lifetime-Based Tumor Contrast Enhancement Using an EGFR Antibody-Labeled Near-Infrared Fluorophore

Rahul Pal et al.

CLINICAL CANCER RESEARCH (2019)

Add to Collection

Article Chemistry, Physical

Improvement of Photostability and NIR Activity of Cyanine Dye through Nanohybrid Formation: Key Information from Ultrafast Dynamical Studies

Arpan Bera et al.

JOURNAL OF PHYSICAL CHEMISTRY A (2019)

Add to Collection

Article Radiology, Nuclear Medicine & Medical Imaging

Multimodal image-guided surgery of HER2-positive breast cancer using [111In]In-DTPA-trastuzumab-IRDye800CW in an orthotopic breast tumor model

Marion M. Deken et al.

EJNMMI RESEARCH (2019)

Add to Collection

Article Chemistry, Multidisciplinary

A Far-Red Fluorescent DNA Binder for Interaction Studies of Live Multidrug-Resistant Pathogens and Host Cells

Leon N. Schulte et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2018)

Add to Collection

Review Chemistry, Multidisciplinary

Far-red fluorescent probes for canonical and non-canonical nucleic acid structures: current progress and future implications

Y. V. Suseela et al.

CHEMICAL SOCIETY REVIEWS (2018)

Add to Collection

Article Chemistry, Organic

Highly homogeneous antibody modification through optimisation of the synthesis and conjugation of functionalised dibromopyridazinediones

Calise Bahou et al.

ORGANIC & BIOMOLECULAR CHEMISTRY (2018)

Add to Collection

Article Chemistry, Organic

NIR-emitting benzothiazolium cyanines with an enhanced stokes shift for mitochondria imaging in live cells

Chathura S. Abeywickrama et al.

ORGANIC & BIOMOLECULAR CHEMISTRY (2018)

Add to Collection

Review Biochemistry & Molecular Biology

Amide Activation in Ground and Excited States

Ervin Kovacs et al.

MOLECULES (2018)

Add to Collection

Article Chemistry, Multidisciplinary

Synthesis and fluorescent properties of boroisoquinolines, a new family of fluorophores

Denes Sovari et al.

RSC ADVANCES (2018)

Add to Collection

Article Medicine, Research & Experimental

Antigen-responsive molecular sensor enables real-time tumor-specific imaging

Hyunjin Kim et al.

THERANOSTICS (2017)

Add to Collection

Article Chemistry, Multidisciplinary

Iodinated cyanine dyes: a new class of sensitisers for use in NIR activated photodynamic therapy (PDT)

Jordan Atchison et al.

CHEMICAL COMMUNICATIONS (2017)

Add to Collection

Article Chemistry, Organic

Cyanine fluorophores for cellular protection against ROS in stimulated macrophages and two-photon ROS detection

M. S. Chan et al.

ORGANIC & BIOMOLECULAR CHEMISTRY (2015)

Add to Collection

Review Chemistry, Organic

Cyanine polyene reactivity: scope and biomedical applications

Alexander P. Gorka et al.

ORGANIC & BIOMOLECULAR CHEMISTRY (2015)

Add to Collection

Review Oncology

Antibody-based imaging strategies for cancer

Jason M. Warram et al.

CANCER AND METASTASIS REVIEWS (2014)

Add to Collection

Article Chemistry, Organic

Synthesis and evaluation of cyanine-styryl dyes with enhanced photostability for fluorescent DNA staining

Peggy R. Bohlaender et al.

ORGANIC & BIOMOLECULAR CHEMISTRY (2013)

Add to Collection

Article Oncology

In vivo breast cancer characterization imaging using two monoclonal antibodies activatably labeled with near infrared fluorophores

Kohei Sano et al.

BREAST CANCER RESEARCH (2012)

Add to Collection

Article Chemistry, Multidisciplinary

An Effective Minor Groove Binder as a Red Fluorescent Marker for Live-Cell DNA Imaging and Quantification

Xiaojun Peng et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2011)

Add to Collection

Review Engineering, Biomedical

A review of NIR dyes in cancer targeting and imaging

Shenglin Luo et al.

BIOMATERIALS (2011)

Add to Collection

Review Radiology, Nuclear Medicine & Medical Imaging

Towards detecting the HER-2 receptor and metabolic changes induced by HER-2-targeted therapies using medical imaging

T. A. D. Smith

BRITISH JOURNAL OF RADIOLOGY (2010)

Add to Collection

Review Oncology

The use of fluorescent dyes and probes in surgical oncology

E. A. te Velde et al.

EJSO (2010)

Add to Collection

Article Chemistry, Physical

A Quantitative Scale for the Extent of Conjugation of Substituted Olefines

Zoltan Mucsi et al.

JOURNAL OF PHYSICAL CHEMISTRY A (2009)

Add to Collection

Article Radiology, Nuclear Medicine & Medical Imaging

Dual-labeled trastuzumab-based imaging agent for the detection of human epidermal growth factor receptor 2 overexpression in breast cancer

Lakshmi Sampath et al.

JOURNAL OF NUCLEAR MEDICINE (2007)

Add to Collection

Article Chemistry, Physical

A quantitative scale for the degree of aromaticity and antiaromaticity: A comparison of theoretical and experimental enthalpies of hydrogenation

Zoltan Musci et al.

JOURNAL OF PHYSICAL CHEMISTRY A (2007)

Add to Collection

© Peeref 2019-2024. All rights reserved.