4.4 Article

Mitochondrial Dynamics Disturbances in the Liver of Broilers with Pulmonary Hypertension Syndrome

Journal

PAKISTAN VETERINARY JOURNAL
Volume 43, Issue 3, Pages 537-544

Publisher

UNIV AGRICULTURE, FAC VETERINARY SCIENCE
DOI: 10.29261/pakvetj/2023.073

Keywords

Broilers; Liver; Mitochondrial dynamics; Mitochondrial fusion and; fission; Pulmonary hypertension; syndrome

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Pulmonary hypertension syndrome (PHS) is a poultry disease that causes economic losses in broilers, and its mechanism is not fully understood. This study found that mitochondrial fusion in the liver was diminished and mitochondrial fission was enhanced in PHS broilers.
Pulmonary hypertension syndrome (PHS) is a poultry disease that causes significant economic losses in broilers. Portal hypertension and liver cirrhosis can cause ascites formation. Disturbance of mitochondrial dynamics causes several diseases; however, its mechanism of action in PHS is not yet fully understood. In this research, 210 broilers were split randomly into two groups: control and PHS. Broilers in the control group were injected with normal saline and those in the PHS group were injected with CM-32 cellulose particles at 20 days of age. Transmission electron microscopy (TEM) was used to assess mitochondrial ultrastructure. Changes in mitochondrial dynamics were measured by immunohistochemical staining, RT-PCR and western blotting. On days 28 and 42, the liver and heart were collected from each group, and the ascites heart index (AHI) and hepatic coefficient were calculated. The results showed that PHS induced hepatocyte damage, reduced mitochondrial size, and increased mitochondrial number. Furthermore, RT-PCR demonstrated the downregulation of Mfn1, Mfn2, and Opa1 mRNA, and the upregulation of Drp1 and Mff mRNA. Protein expression levels detected by immunohistochemistry and western blotting were similar to those obtained by RTPCR. This study showed that mitochondrial fusion in the liver was diminished and mitochondrial fission in the liver was enhanced in PHS broilers.

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