4.7 Article

Predicting metabolic fluxes from omics data via machine learning: Moving from knowledge-driven towards data-driven approaches

Journal

COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
Volume 21, Issue -, Pages 4960-4973

Publisher

ELSEVIER
DOI: 10.1016/j.csbj.2023.10.002

Keywords

Systems biology; Genome-scale models; Metabolic fluxes; Flux balance analysis; Supervised machine learning; Omics data

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Accurate prediction of phenotypes in microorganisms is a major challenge in systems biology. Genome-scale models and constraint-based modeling methods are commonly used for predicting metabolic fluxes, but they require prior knowledge of the metabolic network and appropriate objective functions, limiting their applicability under different conditions. Integrating omics data with supervised machine learning models shows promise in improving phenotype predictions.
The accurate prediction of phenotypes in microorganisms is a main challenge for systems biology. Genome-scale models (GEMs) are a widely used mathematical formalism for predicting metabolic fluxes using constraint-based modeling methods such as flux balance analysis (FBA). However, they require prior knowledge of the metabolic network of an organism and appropriate objective functions, often hampering the prediction of metabolic fluxes under different conditions. Moreover, the integration of omics data to improve the accuracy of phenotype predictions in different physiological states is still in its infancy. Here, we present a novel approach for predicting fluxes under various conditions. We explore the use of supervised machine learning (ML) models using transcriptomics and/or proteomics data and compare their performance against the standard parsimonious FBA (pFBA) approach using case studies of Escherichia coli organism as an example. Our results show that the proposed omics-based ML approach is promising to predict both internal and external metabolic fluxes with smaller prediction errors in comparison to the pFBA approach. The code, data, and detailed results are available at the project's repository [1].

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