Protocol for the isolation of mouse muscle stem cells using fluorescence-activated cell sorting

Article Cell Biology

Prolonged FOS activity disrupts a global myogenic transcriptional program by altering 3D chromatin architecture in primary muscle progenitor cells

A. Rasim Barutcu et al.

Summary: The study reveals that elevated FOS activity in muscle progenitor cells severely hinders cellular differentiation and myotube formation, while also altering chromatin organization near pro-myogenic genes. This highlights the crucial importance of tightly controlling FOS expression in the muscle lineage.

SKELETAL MUSCLE (2022)

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Article Cell Biology

FOS licenses early events in stem cell activation driving skeletal muscle regeneration

Albert E. Almada et al.

Summary: This study identified a prominent Fos mRNA and protein signature in recently activated muscle satellite cells (SCs) that is induced rapidly, heterogeneously, and transiently by muscle damage. The research further revealed the essential role of FOS in initiating key stem cell functions, including cell cycle entry, proliferative expansion, and muscle regeneration, through induction of pro-regenerative genes. Disruption of one of these Fos/AP-1 targets, Art1, delayed cell cycle entry and impeded progenitor cell expansion and muscle regeneration.

CELL REPORTS (2021)

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Article Cell Biology

Established cell surface markers efficiently isolate highly overlapping populations of skeletal muscle satellite cells by fluorescence-activated cell sorting

Claire C. Maesner et al.

SKELETAL MUSCLE (2016)

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Article Biochemical Research Methods

Isolation of skeletal muscle stem cells by fluorescence-activated cell sorting

Ling Liu et al.

NATURE PROTOCOLS (2015)

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Article Biochemistry & Molecular Biology

Highly efficient, functional engraftment of skeletal muscle stem cells in dystrophic muscles

Massimiliano Cerletti et al.

CELL (2008)

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Article Biochemistry & Molecular Biology

Isolation of adult mouse myogenic progenitors: Functional heterogeneity of cells within and engrafting skeletal muscle

RI Sherwood et al.

CELL (2004)

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Protocol for the isolation of mouse muscle stem cells using fluorescence-activated cell sorting

Related references

Prolonged FOS activity disrupts a global myogenic transcriptional program by altering 3D chromatin architecture in primary muscle progenitor cells

FOS licenses early events in stem cell activation driving skeletal muscle regeneration

Established cell surface markers efficiently isolate highly overlapping populations of skeletal muscle satellite cells by fluorescence-activated cell sorting

Isolation of skeletal muscle stem cells by fluorescence-activated cell sorting

Highly efficient, functional engraftment of skeletal muscle stem cells in dystrophic muscles

Isolation of adult mouse myogenic progenitors: Functional heterogeneity of cells within and engrafting skeletal muscle

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