DBP-FP change of biofilm in drinking water distribution system induced by sequential UV and chlorine disinfection: Effect of UV dose and influencing mechanism

The issue of biofilm-related disinfection byproducts (DBPs) in drinking water distribution system (DWDS) has garnered significant attention. This study sought to examine the changes in biofilm-originated halogenated DBP formation potential (biofilm DBP-FP) in simulated continuous-flow DWDSs subjected to sequential UV and chlorine disinfection (UV-Cl2) treatments with varying UV doses and to propose the underlying mechanism. The formation potential of trihalomethanes (THMs), haloacetic acids (HAAs), and the total organic halogen (TOX, X = Cl and Br) produced by biofilm were measured. Results showed that the biofilm TOCl-FP was at a minimum with a UV dose of 80 mJ/cm2, corresponding to the lowest amounts of protein and polysaccharides in the extracellular polymeric substances (EPS). Sphingobium, Methylobacterium, and Sphingomonas played a crucial role in protein and polysaccharide biosynthesis. Bacterial community composition characterization together with metabolic function analysis indicated that dominant bacteria varied and metabolic function shifted due to UV-Cl2 disinfection, with Alphaproteobacteria increasing in relative abundance and Bacteroidia showing the opposite trend with increasing UV doses. Correlation analysis suggested that the UV-Cl2 disinfection process led to changes in the water matrix, including organics, inorganics, bacteria, and components that provide environ -mental pressure for the biofilm. These changes ultimately influenced the properties of the biofilm EPS, which had a direct impact on biofilm DBP-FP.

Automated summary

This study investigated the changes in biofilm DBP formation potential in continuous-flow DWDS subjected to UV-Cl2 treatments. The results showed that the minimum biofilm DBP formation potential was observed at a UV dose of 80 mJ/cm2, and the bacterial community composition and metabolic function were also affected by UV-Cl2 treatment. Additionally, the UV-Cl2 disinfection process led to changes in the water matrix, which influenced the properties of biofilm EPS and DBP formation potential.