Journal
BIOTECHNOLOGY JOURNAL
Volume 11, Issue 10, Pages 1268-1273Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/biot.201600266
Keywords
Cell wall structure; Lignin; S/G ratio; Thioacidolysis
Funding
- Office of Biological and Environmental Research in the US Department of Energy Office of Science [DE-AC05-00OR22725]
- Oak Ridge National Laboratory [DE-AC36-08-GO28308]
- National Renewable Energy Laboratory
- DOE Great Lakes Bioenergy Research Center (DOE Office of Science BER) [DE-FC02-07ER64494]
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Thioacidolysis is a method used to measure the relative content of lignin monomers bound by beta-O-4 linkages. Current thioacidolysis methods are low-throughput as they require tedious steps for reaction product concentration prior to analysis using standard GC methods. A quantitative thioacidolysis method that is accessible with general laboratory equipment and uses a non-chlorinated organic solvent and is tailored for higher-throughput analysis is reported. The method utilizes lignin arylglycerol monomer standards for calibration, requires 1-2 mg of biomass per assay and has been quantified using fast-GC techniques including a Low Thermal Mass Modular Accelerated Column Heater (LTM MACH). Cumbersome steps, including standard purification, sample concentrating and drying have been eliminated to help aid in consecutive day-to-day analyses needed to sustain a high sample throughput for large screening experiments without the loss of quantitation accuracy. The method reported in this manuscript has been quantitatively validated against a commonly used thioacidolysis method and across two different research sites with three common biomass varieties to represent hardwoods, softwoods, and grasses.
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