Journal
BIOTECHNOLOGY JOURNAL
Volume 11, Issue 8, Pages 1000-1013Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/biot.201500603
Keywords
DNA solubility; Enzyme activity; Ionic liquid; Microorganism; Protein stability
Funding
- Centre of Research in Ionic Liquids (CORIL), Universiti Teknologi PETRONAS
- Grants-in-Aid for Scientific Research [16H06369] Funding Source: KAKEN
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The technological utility of biomolecules (e.g. proteins, enzymes and DNA) can be significantly enhanced by combining them with ionic liquids (ILs) - potentially attractive green and designer solvents - rather than using in conventional organic solvents or water. In recent years, ILs have been used as solvents, cosolvents, and reagents for biocatalysis, biotransformation, protein preservation and stabilization, DNA solubilization and stabilization, and other biomolecule-based applications. Using ILs can dramatically enhance the structural and chemical stability of proteins, DNA, and enzymes. This article reviews the recent technological developments of ILs in protein-, enzyme-, and DNA-based applications. We discuss the different routes to increase biomolecule stability and activity in ILs, and the design of biomolecule-friendly ILs that can dissolve biomolecules with minimum alteration to their structure. This information will be helpful to design IL-based processes in biotechnology and the biological sciences that can serve as novel and selective processes for enzymatic reactions, protein and DNA stability, and other biomolecule-based applications.
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